CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017007
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho guanine nucleotide exchange factor 19 
Protein Synonyms/Alias
 Ephexin-2 
Gene Name
 ARHGEF19 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
423DKQWLFSKLPEVKSTacetylation[1]
428FSKLPEVKSTSERFLacetylation[1]
763VRLADGEKGWVPQAYubiquitination[2]
796RVTSATSKLGEAPV*ubiquitination[2, 3]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase (By similarity). 
Sequence Annotation
 DOMAIN 376 560 DH.
 DOMAIN 592 704 PH.
 DOMAIN 715 776 SH3.  
Keyword
 Alternative splicing; Complete proteome; GTPase activation; Guanine-nucleotide releasing factor; Polymorphism; Reference proteome; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 802 AA 
Protein Sequence
MDCGPPATLQ PHLTGPPGTA HHPVAVCQQE SLSFAELPAL KPPSPVCLDL FPVAPEELRA 60
PGSRWSLGTP APLQGLLWPL SPGGSDTEIT SGGMRPSRAG SWPHCPGAQP PALEGPWSPR 120
HTQPQRRASH GSEKKSAWRK MRVYQREEVP GCPEAHAVFL EPGQVVQEQA LSTEEPRVEL 180
SGSTRVSLEG PERRRFSASE LMTRLHSSLR LGRNSAARAL ISGSGTGAAR EGKASGMEAR 240
SVEMSGDRVS RPAPGDSREG DWSEPRLDTQ EEPPLGSRST NERRQSRFLL NSVLYQEYSD 300
VASARELRRQ QREEEGPGDE AEGAEEGPGP PRANLSPSSS FRAQRSARGS TFSLWQDIPD 360
VRGSGVLATL SLRDCKLQEA KFELITSEAS YIHSLSVAVG HFLGSAELSE CLGAQDKQWL 420
FSKLPEVKST SERFLQDLEQ RLEADVLRFS VCDVVLDHCP AFRRVYLPYV TNQAYQERTY 480
QRLLLENPRF PGILARLEES PVCQRLPLTS FLILPFQRIT RLKMLVENIL KRTAQGSEDE 540
DMATKAFNAL KELVQECNAS VQSMKRTEEL IHLSKKIHFE GKIFPLISQA RWLVRHGELV 600
ELAPLPAAPP AKLKLSSKAV YLHLFNDCLL LSRRKELGKF AVFVHAKMAE LQVRDLSLKL 660
QGIPGHVFLL QLLHGQHMKH QFLLRARTES EKQRWISALC PSSPQEDKEV ISEGEDCPQV 720
QCVRTYKALH PDELTLEKTD ILSVRTWTSD GWLEGVRLAD GEKGWVPQAY VEEISSLSAR 780
LRNLRENKRV TSATSKLGEA PV 802 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0032321; P:positive regulation of Rho GTPase activity; IEA:Compara.
 GO:0032956; P:regulation of actin cytoskeleton organization; IGI:MGI.
 GO:0042060; P:wound healing; IEA:Compara. 
Interpro
 IPR000219; DH-domain.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR001452; SH3_domain. 
Pfam
 PF00621; RhoGEF
 PF00018; SH3_1 
SMART
 SM00233; PH
 SM00325; RhoGEF
 SM00326; SH3 
PROSITE
 PS00741; DH_1
 PS50010; DH_2
 PS50003; PH_DOMAIN
 PS50002; SH3 
PRINTS