CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-040139
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Insulin-degrading enzyme 
Protein Synonyms/Alias
  
Gene Name
 Ide 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
160AVDSEHEKNVMNDAWacetylation[1, 2, 3, 4, 5]
160AVDSEHEKNVMNDAWsuccinylation[4]
160AVDSEHEKNVMNDAWubiquitination[6]
174WRLFQLEKATGNPKHubiquitination[6]
276LYKIVPIKDIRNLYVacetylation[2]
276LYKIVPIKDIRNLYVubiquitination[6]
368HMFQYIQKLRAEGPQacetylation[7]
404RPRGYTSKIAGKLHYacetylation[1, 4, 5]
404RPRGYTSKIAGKLHYsuccinylation[4]
404RPRGYTSKIAGKLHYubiquitination[6]
451VRVAIVSKSFEGKTDacetylation[1]
456VSKSFEGKTDRTEQWacetylation[4]
456VSKSFEGKTDRTEQWsuccinylation[4]
510FEILSLEKDATPYPAubiquitination[6]
526IKDTAMSKLWFKQDDubiquitination[6]
631KKRFEIIKEAYMRSLacetylation[4]
631KKRFEIIKEAYMRSLsuccinylation[4]
665MTEVAWTKDELKEALacetylation[4]
665MTEVAWTKDELKEALsuccinylation[4]
681DVTLPRLKAFIPQLLacetylation[1]
681DVTLPRLKAFIPQLLubiquitination[6]
794CFNTLRTKEQLGYIVubiquitination[6]
852MTEEAFQKHIQALAIubiquitination[6]
901AYLKTLTKDDIIRFYubiquitination[6]
986HINFMAAKL******ubiquitination[6]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [6] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [7] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 987 AA 
Protein Sequence
XFPKQTYSTM SNPAIQRIED QIVKSPEDKR EYRGLELANG IKVLLISDPT TDKSSAALDV 60
HIGSLSDPPN IPGLSHFCEH MLFLGTKKYP KENEYSQFLS EHAGSSNAFT SGEHTNYYFD 120
VSHEHLEGAL DRFAQFFLCP LFDASCKDRE VNAVDSEHEK NVMNDAWRLF QLEKATGNPK 180
HPFSKFGTGN KYTLETRPNQ EGIDVREELL KFHSTYYSSN LMAICVLGRE SLDDLTNLVV 240
KLFSEVENKN VPLPEFPEHP FQEEHLRQLY KIVPIKDIRN LYVTFPIPDL QQYYKSNPGH 300
YLGHLIGHEG PGSLLSELKS KGWVNTLVGG QKEGARGFMF FIINVDLTEE GLLHVEDIIL 360
HMFQYIQKLR AEGPQEWVFQ ECKDLNAVAF RFKDKERPRG YTSKIAGKLH YYPLNGVLTA 420
EYLLEEFRPD LIDMVLDKLR PENVRVAIVS KSFEGKTDRT EQWYGTQYKQ EAIPEDIIQK 480
WQNADLNGKF KLPTKNEFIP TNFEILSLEK DATPYPALIK DTAMSKLWFK QDDKFFLPKA 540
CLNFEFFSPF AYVDPLHCNM AYLYLELLKD SLNEYAYAAE LAGLSYDLQN TIYGMYLSVK 600
GYNDKQPILL KKITEKMATF EIDKKRFEII KEAYMRSLNN FRAEQPHQHA MYYLRLLMTE 660
VAWTKDELKE ALDDVTLPRL KAFIPQLLSR LHIEALLHGN ITKQAALGVM QMVEDTLIEH 720
AHTKPLLPSQ LVRYREVQLP DRGWFVYQQR NEVHNNCGIE IYYQTDMQST SENMFLELFC 780
QIISEPCFNT LRTKEQLGYI VFSGPRRANG IQGLRFIIQS EKPPHYLESR VEAFLITMEK 840
AIEDMTEEAF QKHIQALAIR RLDKPKKLSA ECAKYWGEII SQQYNYDRDN IEVAYLKTLT 900
KDDIIRFYQE MLAVDAPRRH KVSVHVLARE MDSCPVVGEF PSQNDINLSE APPLPQPEVI 960
HNMTEFKRGL PLFPLVKPHI NFMAAKL 987 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0046872; F:metal ion binding; IEA:InterPro.
 GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
 GO:0006508; P:proteolysis; IEA:InterPro. 
Interpro
 IPR011249; Metalloenz_LuxS/M16.
 IPR011237; Pept_M16_dom.
 IPR011765; Pept_M16_N.
 IPR001431; Pept_M16_Zn_BS.
 IPR007863; Peptidase_M16_C. 
Pfam
 PF00675; Peptidase_M16
 PF05193; Peptidase_M16_C 
SMART
  
PROSITE
 PS00143; INSULINASE 
PRINTS