UniProt Accession

 ENOPH_MOUSE; Q8BGB7; Q3TGK8; Q3TXA2; Q3U8Y4; Q8VC92; Q9D6U4 

Genbank Protein ID

 AK009954; AK079062; AK080714; AK082747; AK152021; AK159356; AK167575; AK168696; BC021429 

Genbank Nucleotide ID

 BAB26606.1; BAC37520.1; BAC37988.1; BAC38597.1; BAE30883.1; BAE35014.1; BAE39638.1; BAE40540.1; AAH21429.1 

Protein Name

 Enolase-phosphatase E1 

Protein Synonyms/Alias

 2,3-diketo-5-methylthio-1-phosphopentane phosphatase; MASA homolog 

Gene Name

 Enoph1 

Gene Synonyms/Alias

 Masa 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
36	DVLFPYIKENVKEYL	acetylation	[1]
36	DVLFPYIKENVKEYL	ubiquitination	[2]

Reference

 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) (By similarity). 

Sequence Annotation

 REGION 150 151 Substrate binding (By similarity).
 METAL 16 16 Magnesium (By similarity).
 METAL 18 18 Magnesium; via carbonyl oxygen (By
 METAL 209 209 Magnesium (By similarity).
 BINDING 184 184 Substrate (By similarity).  

Keyword

 Alternative splicing; Amino-acid biosynthesis; Complete proteome; Cytoplasm; Hydrolase; Magnesium; Metal-binding; Methionine biosynthesis; Nucleus; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 257 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0043874; F:acireductone synthase activity; ISS:UniProtKB.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0008967; F:phosphoglycolate phosphatase activity; IEA:InterPro.
 GO:0019509; P:L-methionine salvage from methylthioadenosine; ISS:UniProtKB. 

Interpro

 IPR023943; Enolase-ppase_E1.
 IPR027511; ENOPH1_eukaryotes.
 IPR023214; HAD-like_dom.
 IPR006439; HAD-SF_hydro_IA_v1. 

Pfam

 PF13419; HAD_2 

SMART

  

PROSITE

  

PRINTS