CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017017
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein DENND6A 
Protein Synonyms/Alias
 DENN domain-containing protein 6A 
Gene Name
 DENND6A 
Gene Synonyms/Alias
 FAM116A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
93SKLTDREKTNICYLSubiquitination[1]
360VTNPFFAKTLQHWPHubiquitination[1]
375IIRIGDLKPTGEIPKubiquitination[1, 2]
463ASLMPLQKSISPWKSubiquitination[1]
469QKSISPWKSPPQLRQubiquitination[1]
484FLPEEFMKTLEKTGPubiquitination[1, 2]
488EFMKTLEKTGPQLTSubiquitination[1]
498PQLTSRIKGDWIGLYubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Guanine nucleotide exchange factor (GEF) for RAB14. Component of an endocytic recycling pathway that is required for the control of ADAM10 transport, shedding of N-cadherin/CDH2 by ADAM9 or ADAM10 and regulation of cell-cell junctions. Required for RAB14 recruitment to recycling endosomes. 
Sequence Annotation
 MOD_RES 127 127 Phosphoserine.  
Keyword
 Complete proteome; Cytoplasm; Endosome; Guanine-nucleotide releasing factor; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 608 AA 
Protein Sequence
MALRGPAGLG PGSRRPLDEA VAGAEGREAP ALVAAGGAPE DDEEDDGRGR GLLRWDSFSA 60
WLHCVCVVGF DLELGQAVEV IYPQHSKLTD REKTNICYLS FPDSNSGCLG DTQFCFRFRQ 120
SSGRRVSLHC LLDQFDKDLP VYLKKDPAYF YGYVYFRQVR DKTLKRGYFQ KSLVLISKLP 180
YIHFFHTVLK QIAPEYFEKN EPYLEAACND VDRWPAPVPG KTLHLPIMGV VMKVRIPTCH 240
DKPGTTQIVQ LTQQVDTNIS VILPTVHEVD IFRCFCPVFL HSQMLWELVL LGEPLVVMAP 300
SPSESSETVL ALVNCISPLK YFSDFRPYFT IHDSEFKEYT TRTQAPPSVI LGVTNPFFAK 360
TLQHWPHIIR IGDLKPTGEI PKQVKVKKLK NLKTLDSKPG VYTSYKPYLN RDEEIIKQLQ 420
KGVQQKRPSE AQSVILRRYF LELTQSFIIP LERYVASLMP LQKSISPWKS PPQLRQFLPE 480
EFMKTLEKTG PQLTSRIKGD WIGLYRHFLK SPNFDGWFKT RRKEMTQKLE ALHLEALCEE 540
DLLLWIQKHT EVETVDLVLK LKNKLLQADR EHLPVKPDTM EKLRTHIDAI ILALPEDLQG 600
ILLKTGMT 608 
Gene Ontology
 GO:0055037; C:recycling endosome; IDA:UniProtKB.
 GO:0017112; F:Rab guanyl-nucleotide exchange factor activity; IDA:UniProtKB.
 GO:2000049; P:positive regulation of cell-cell adhesion mediated by cadherin; IDA:UniProtKB. 
Interpro
 IPR012860; Afi1_N.
 IPR024224; Uncharacterised_FAM116. 
Pfam
 PF07792; Afi1 
SMART
  
PROSITE
  
PRINTS