CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000382
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Secretory carrier-associated membrane protein 1 
Protein Synonyms/Alias
 Secretory carrier membrane protein 1 
Gene Name
 SCAMP1 
Gene Synonyms/Alias
 SCAMP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
52TPPPGGVKMPNVPNTubiquitination[1, 2]
65NTQPAIMKPTEEHPAubiquitination[1, 2, 3, 4]
78PAYTQIAKEHALAQAubiquitination[1, 2, 3]
89LAQAELLKRQEELERubiquitination[1, 2, 3, 4, 5, 6, 7, 8, 9]
97RQEELERKAAELDRRubiquitination[7]
298TTGASFEKAQQEFATubiquitination[1, 2, 4, 5, 7]
311ATGVMSNKTVQTAAAubiquitination[1, 2, 3, 5]
334SAAQNAFKGNQI***ubiquitination[1, 2, 3, 4, 5, 6, 7, 8, 9]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface. 
Sequence Annotation
 MOD_RES 2 2 N-acetylserine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Endosome; Golgi apparatus; Membrane; Protein transport; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 338 AA 
Protein Sequence
MSDFDSNPFA DPDLNNPFKD PSVTQVTRNV PPGLDEYNPF SDSRTPPPGG VKMPNVPNTQ 60
PAIMKPTEEH PAYTQIAKEH ALAQAELLKR QEELERKAAE LDRREREMQN LSQHGRKNNW 120
PPLPSNFPVG PCFYQDFSVD IPVEFQKTVK LMYYLWMFHA VTLFLNIFGC LAWFCVDSAR 180
AVDFGLSILW FLLFTPCSFV CWYRPLYGAF RSDSSFRFFV FFFVYICQFA VHVLQAAGFH 240
NWGNCGWISS LTGLNQNIPV GIMMIIIAAL FTASAVISLV MFKKVHGLYR TTGASFEKAQ 300
QEFATGVMSN KTVQTAAANA ASTAASSAAQ NAFKGNQI 338 
Gene Ontology
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IDA:UniProtKB.
 GO:0055038; C:recycling endosome membrane; IDA:UniProtKB.
 GO:0005802; C:trans-Golgi network; IDA:UniProtKB.
 GO:0006892; P:post-Golgi vesicle-mediated transport; TAS:ProtInc.
 GO:0015031; P:protein transport; IDA:UniProtKB. 
Interpro
 IPR007273; SCAMP. 
Pfam
 PF04144; SCAMP 
SMART
  
PROSITE
  
PRINTS