UniProt Accession

 CP2E1_MOUSE; Q05421; Q9Z198 

Genbank Protein ID

 L11650; X62595; BC013451; M54877; X01026 

Genbank Nucleotide ID

 AAA39879.1; CAA44481.1; AAH13451.1; AAA37275.1; CAA25510.1 

Protein Name

 Cytochrome P450 2E1 

Protein Synonyms/Alias

 4-nitrophenol 2-hydroxylase; CYPIIE1; Cytochrome P450-ALC; Cytochrome P450-J 

Gene Name

 Cyp2e1 

Gene Synonyms/Alias

 Cyp2e; Cyp2e-1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
55	LDLKDIPKSLTKLAK	ubiquitination	[1]
84	IVVLHGYKAVKEVLL	ubiquitination	[1]
87	LHGYKAVKEVLLNHK	ubiquitination	[1]
94	KEVLLNHKNEFSGRG	acetylation	[2]
94	KEVLLNHKNEFSGRG	ubiquitination	[1]
110	IPVFQEYKNKGIIFN	acetylation	[3]
110	IPVFQEYKNKGIIFN	ubiquitination	[1]
112	VFQEYKNKGIIFNNG	ubiquitination	[1]
123	FNNGPTWKDVRRFSL	ubiquitination	[1]
140	LRDWGMGKQGNEARI	ubiquitination	[1]
159	HFLVEELKKTKGQPF	acetylation	[2]
159	HFLVEELKKTKGQPF	ubiquitination	[1]
160	FLVEELKKTKGQPFD	ubiquitination	[1]
194	KRFDYDDKKCLELMS	acetylation	[2]
194	KRFDYDDKKCLELMS	ubiquitination	[1]
234	YLPGSHRKVMKNVSE	ubiquitination	[1]
237	GSHRKVMKNVSEIRQ	ubiquitination	[1]
249	IRQYTLGKAKEHLKS	ubiquitination	[1]
251	QYTLGKAKEHLKSLD	ubiquitination	[1]
255	GKAKEHLKSLDINCP	ubiquitination	[1]
275	CLLIEMEKEKHSQEP	ubiquitination	[1]
324	KYPEIEEKLHEEIDR	ubiquitination	[1]
422	LNENGKFKYSDYFKA	ubiquitination	[1]
428	FKYSDYFKAFSAGKR	ubiquitination	[1]
434	FKAFSAGKRVCVGEG	ubiquitination	[1]
467	LKSLVDPKDIDLSPV	ubiquitination	[1]
486	GSIPREFKLCVIPRS	acetylation	[3]
486	GSIPREFKLCVIPRS	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites (By similarity). 

Sequence Annotation

 REGION 298 303 Substrate binding (By similarity).
 METAL 437 437 Iron (heme axial ligand) (By similarity).
 MOD_RES 129 129 Phosphoserine.  

Keyword

 Complete proteome; Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome; Monooxygenase; NADP; Oxidoreductase; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 493 AA 

Protein Sequence

Gene Ontology

 GO:0005783; C:endoplasmic reticulum; IDA:MGI.
 GO:0000139; C:Golgi membrane; IEA:Compara.
 GO:0031227; C:intrinsic to endoplasmic reticulum membrane; IEA:Compara.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0020037; F:heme binding; ISS:UniProtKB.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0004497; F:monooxygenase activity; IDA:MGI.
 GO:0016709; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen; IEA:EC.
 GO:0016712; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; IEA:Compara.
 GO:0017144; P:drug metabolic process; IEA:Compara.
 GO:0046483; P:heterocycle metabolic process; IEA:Compara.
 GO:0016098; P:monoterpenoid metabolic process; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0045471; P:response to ethanol; IEA:Compara.
 GO:0010243; P:response to organic nitrogen; IEA:Compara.
 GO:0010193; P:response to ozone; IEA:Compara.
 GO:0008202; P:steroid metabolic process; IEA:Compara.
 GO:0006641; P:triglyceride metabolic process; IEA:Compara. 

Interpro

 IPR001128; Cyt_P450.
 IPR017972; Cyt_P450_CS.
 IPR002401; Cyt_P450_E_grp-I.
 IPR008070; Cyt_P450_E_grp-I_CYP2E-like. 

Pfam

 PF00067; p450 

SMART

  

PROSITE

 PS00086; CYTOCHROME_P450 

PRINTS

 PR00463; EP450I.
 PR01687; EP450ICYP2E.
 PR00385; P450.