CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023968
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Solute carrier family 12 member 7 
Protein Synonyms/Alias
 Electroneutral potassium-chloride cotransporter 4; K-Cl cotransporter 4 
Gene Name
 SLC12A7 
Gene Synonyms/Alias
 KCC4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
393AGAFVEKKGVPSVPVubiquitination[1]
759LMSTEKTKGFCQLVVubiquitination[1]
893DNSIQMKKDLQMFLYubiquitination[2]
937EQRSQMLKQMQLSKNubiquitination[1, 2, 3]
943LKQMQLSKNEQEREAubiquitination[1, 3]
976QAPPTPDKVQMTWTRubiquitination[1, 2, 3, 4, 5]
990REKLIAEKYRSRDTSubiquitination[1, 3, 6]
1002DTSLSGFKDLFSMKPubiquitination[2, 3, 5]
1008FKDLFSMKPDQSNVRubiquitination[1, 2, 3, 4, 5, 7]
1030LNGVVLNKSQDAQLVubiquitination[1]
1046LNMPGPPKNRQGDENubiquitination[1, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification (By similarity). 
Sequence Annotation
 MOD_RES 50 50 Phosphoserine.
 MOD_RES 62 62 Phosphoserine.
 MOD_RES 926 926 Phosphothreonine (By similarity).
 MOD_RES 980 980 Phosphothreonine (By similarity).
 CARBOHYD 312 312 N-linked (GlcNAc...) (high mannose).
 CARBOHYD 331 331 N-linked (GlcNAc...).
 CARBOHYD 344 344 N-linked (GlcNAc...).
 CARBOHYD 360 360 N-linked (GlcNAc...) (high mannose).  
Keyword
 Alternative splicing; Cell membrane; Complete proteome; Glycoprotein; Ion transport; Membrane; Phosphoprotein; Polymorphism; Potassium; Potassium transport; Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1083 AA 
Protein Sequence
MPTNFTVVPV EAHADGGGDE TAERTEAPGT PEGPEPERPS PGDGNPRENS PFLNNVEVEQ 60
ESFFEGKNMA LFEEEMDSNP MVSSLLNKLA NYTNLSQGVV EHEEDEESRR REAKAPRMGT 120
FIGVYLPCLQ NILGVILFLR LTWIVGVAGV LESFLIVAMC CTCTMLTAIS MSAIATNGVV 180
PAGGSYYMIS RSLGPEFGGA VGLCFYLGTT FAGAMYILGT IEIFLTYISP GAAIFQAEAA 240
GGEAAAMLHN MRVYGTCTLV LMALVVFVGV KYVNKLALVF LACVVLSILA IYAGVIKSAF 300
DPPDIPVCLL GNRTLSRRSF DACVKAYGIH NNSATSALWG LFCNGSQPSA ACDEYFIQNN 360
VTEIQGIPGA ASGVFLENLW STYAHAGAFV EKKGVPSVPV AEESRASALP YVLTDIAASF 420
TLLVGIYFPS VTGIMAGSNR SGDLKDAQKS IPTGTILAIV TTSFIYLSCI VLFGACIEGV 480
VLRDKFGEAL QGNLVIGMLA WPSPWVIVIG SFFSTCGAGL QSLTGAPRLL QAIARDGIVP 540
FLQVFGHGKA NGEPTWALLL TVLICETGIL IASLDSVAPI LSMFFLMCYL FVNLACAVQT 600
LLRTPNWRPR FKFYHWTLSF LGMSLCLALM FICSWYYALS AMLIAGCIYK YIEYRGAEKE 660
WGDGIRGLSL NAARYALLRV EHGPPHTKNW RPQVLVMLNL DAEQAVKHPR LLSFTSQLKA 720
GKGLTIVGSV LEGTYLDKHM EAQRAEENIR SLMSTEKTKG FCQLVVSSSL RDGMSHLIQS 780
AGLGGLKHNT VLMAWPASWK QEDNPFSWKN FVDTVRDTTA AHQALLVAKN VDSFPQNQER 840
FGGGHIDVWW IVHDGGMLML LPFLLRQHKV WRKCRMRIFT VAQVDDNSIQ MKKDLQMFLY 900
HLRISAEVEV VEMVENDISA FTYERTLMME QRSQMLKQMQ LSKNEQEREA QLIHDRNTAS 960
HTAAAARTQA PPTPDKVQMT WTREKLIAEK YRSRDTSLSG FKDLFSMKPD QSNVRRMHTA 1020
VKLNGVVLNK SQDAQLVLLN MPGPPKNRQG DENYMEFLEV LTEGLNRVLL VRGGGREVIT 1080
IYS 1083 
Gene Ontology
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0015379; F:potassium:chloride symporter activity; TAS:ProtInc.
 GO:0006884; P:cell volume homeostasis; IEA:Compara.
 GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW. 
Interpro
 IPR004841; AA-permease_dom.
 IPR000076; KCL_cotranspt.
 IPR004842; Na/K/Cl_cotransptS. 
Pfam
 PF00324; AA_permease 
SMART
  
PROSITE
  
PRINTS
 PR01081; KCLTRNSPORT.