CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000185
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TFIIH basal transcription factor complex helicase XPD subunit 
Protein Synonyms/Alias
 CXPD; DNA excision repair protein ERCC-2; DNA repair protein complementing XP-D cells; Xeroderma pigmentosum group D-complementing protein 
Gene Name
 Ercc2 
Gene Synonyms/Alias
 Xpd 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
507DQVAISSKFETREDIubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 ATP-dependent 5'-3' DNA helicase, component of the core- TFIIH basal transcription factor. Involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers (By similarity). 
Sequence Annotation
 DOMAIN 7 283 Helicase ATP-binding.
 NP_BIND 42 49 ATP (By similarity).
 REGION 438 637 Mediates interaction with MMS19 (By
 MOTIF 234 237 DEAH box.
 METAL 116 116 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 134 134 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 155 155 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 190 190 Iron-sulfur (4Fe-4S) (By similarity).  
Keyword
 4Fe-4S; ATP-binding; Chromosome partition; Complete proteome; Cytoplasm; Cytoskeleton; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase; Iron; Iron-sulfur; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 760 AA 
Protein Sequence
MKLNVDGLLV YFPYDYIYPE QFSYMLELKR TLDAKGHGVL EMPSGTGKTV SLLALIVAYQ 60
RAYPLEVTKL IYCSRTVPEI EKVIEELRKL LSFYEQQEGE KLPFLGLALS SRKNLCIHPE 120
VTPLRFGKDV DGKCHSLTAS YVRAQYQQDA SLPHCRFYEE FDIHGRQMPL PAGIYNLDDL 180
KALGQRQGWC PYFLARYSIL HANVVVYSYH YLLDPKIADL VSKELARKAV VVFDEAHNID 240
NVCIDSMSVN LTRRTLDRCQ SNLDTLQKTV LRIKETDEQR LRDEYRRLVE GLREASVARE 300
TDAHLANPVL PDEVLQEAVP GSIRTAEHFL GFLRRLLEYV KWRLRVQHVV QESPPAFLSG 360
LAQRVCIQRK PLRFCAERLR SLLHTLEIAD LADFSPLTLL ANFATLVSTY AKGFTIIIEP 420
FDDRTPTIAN PVLHFSCMDA SLAIKPVFER FQSVIITSGT LSPLDIYPKI LDFHPVTMAT 480
FTMTLARVCL CPMIIGRGND QVAISSKFET REDIAVIRNY GNLLLEMSAV VPDGIVAFFT 540
SYQYMESTVA SWYEQGILEN IQRNKLLFIE TQDGAETSVA LEKYQEACEN GRGAILLSVA 600
RGKVSEGIDF VHHYGRAVIM FGVPYVYTQS RILKARLEYL RDQFQIREND FLTFDAMRHA 660
AQCVGRAIRG KTDYGLMVFA DKRFARADKR GKLPRWIQEH LTDSNLNLTV DEGVQVAKYF 720
LRQMAQPFHR EDQLGLSLLS LEQLQSEETL QRIEQIAQQL 760 
Gene Ontology
 GO:0019907; C:cyclin-dependent protein kinase activating kinase holoenzyme complex; IEA:Compara.
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005675; C:holo TFIIH complex; ISS:UniProtKB.
 GO:0071817; C:MMXD complex; ISS:UniProtKB.
 GO:0000441; C:SSL2-core TFIIH complex; IEA:Compara.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0043139; F:5'-3' DNA helicase activity; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004003; F:ATP-dependent DNA helicase activity; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008353; F:RNA polymerase II carboxy-terminal domain kinase activity; IEA:Compara.
 GO:0007568; P:aging; IMP:MGI.
 GO:0006915; P:apoptotic process; IMP:MGI.
 GO:0030282; P:bone mineralization; IMP:MGI.
 GO:0000075; P:cell cycle checkpoint; IEA:Compara.
 GO:0008283; P:cell proliferation; IMP:MGI.
 GO:0032289; P:central nervous system myelin formation; IMP:MGI.
 GO:0007059; P:chromosome segregation; ISS:UniProtKB.
 GO:0040016; P:embryonic cleavage; IMP:MGI.
 GO:0043249; P:erythrocyte maturation; IMP:MGI.
 GO:0030198; P:extracellular matrix organization; IMP:MGI.
 GO:0035315; P:hair cell differentiation; IMP:MGI.
 GO:0048820; P:hair follicle maturation; IMP:MGI.
 GO:0060218; P:hematopoietic stem cell differentiation; IMP:MGI.
 GO:0001701; P:in utero embryonic development; IMP:MGI.
 GO:0006917; P:induction of apoptosis; ISS:UniProtKB.
 GO:0035264; P:multicellular organism growth; IMP:MGI.
 GO:0043066; P:negative regulation of apoptotic process; IMP:MGI.
 GO:0033683; P:nucleotide-excision repair, DNA incision; IEA:Compara.
 GO:0043388; P:positive regulation of DNA binding; IMP:MGI.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0009791; P:post-embryonic development; IMP:MGI.
 GO:0001666; P:response to hypoxia; IEA:Compara.
 GO:0006979; P:response to oxidative stress; IMP:MGI.
 GO:0043588; P:skin development; IMP:MGI.
 GO:0021510; P:spinal cord development; IMP:MGI.
 GO:0006366; P:transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0006283; P:transcription-coupled nucleotide-excision repair; ISS:UniProtKB.
 GO:0009650; P:UV protection; IMP:MGI. 
Interpro
 IPR006555; ATP-dep_Helicase_C.
 IPR010614; DEAD_2.
 IPR002464; DNA/RNA_helicase_DEAH_CS.
 IPR013020; DNA_helicase_DNA-repair_Rad3.
 IPR010643; DUF1227.
 IPR014013; Helic_SF1/SF2_ATP-bd_DinG/Rad3.
 IPR006554; Helicase-like_DEXD_c2.
 IPR027417; P-loop_NTPase.
 IPR001945; XPGD_DNA_repair. 
Pfam
 PF06733; DEAD_2
 PF06777; DUF1227
 PF13307; Helicase_C_2 
SMART
 SM00488; DEXDc2
 SM00491; HELICc2 
PROSITE
 PS00690; DEAH_ATP_HELICASE
 PS51193; HELICASE_ATP_BIND_2 
PRINTS
 PR00852; XRODRMPGMNTD.