CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-040856
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Uridine kinase 
Protein Synonyms/Alias
  
Gene Name
 UCKL1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
66KRTTSQCKSEPPLLRubiquitination[1]
111GGGSASGKTTVARMIubiquitination[1]
179LKQGKSVKVPIYDFTubiquitination[1]
254RDIEGVIKQYNKFVKubiquitination[1, 2]
261KQYNKFVKPSFDQYIubiquitination[1, 3, 4, 5]
328PRTLSVLKSTPQVRGubiquitination[1, 3, 4, 5]
343MHTIIRDKETSRDEFubiquitination[1]
387QGQDYAGKCYAGKQIubiquitination[1, 2]
392AGKCYAGKQITGVSIubiquitination[1, 4, 5]
510IITTAVDKRVNDLFRacetylation[5]
510IITTAVDKRVNDLFRubiquitination[1, 3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 548 AA 
Protein Sequence
MAAPPARADA DPSPTSPPTA RDTPGRQAEK SETACEDRSN AESLDRLLPP VGTGRSPRKR 60
TTSQCKSEPP LLRTSKRTIY TAGRPPWYNE HGTQSKEAFA IGLGGGSASG KTTVARMIIE 120
ALDVPWVVLL SMDSFYKVLT EQQQEQAAHN NFNFDHPDAF DFDLIISTLK KLKQGKSVKV 180
PIYDFTTHSR KKDWKTLYGA NVIIFEGIMA FADKTLLELL DMKIFVDTDS DIRLVRRLRR 240
DISERGRDIE GVIKQYNKFV KPSFDQYIQP TMRLADIVVP RGSGNTVAID LIVQHVHSQL 300
EELRFVPRAA LASAHQCHPL PRTLSVLKST PQVRGMHTII RDKETSRDEF IFYSKRLMRL 360
LIEHALSFLP FQDCVVQTPQ GQDYAGKCYA GKQITGVSIL RAGETMEPAL RAVCKDVRIG 420
TILIQTNQLT GEPELHYLRL PKDISDDHVI LMDCTVSTGA AAMMAVRVLL DHDVPEDKIF 480
LLSLLMAEMG VHSVAYAFPR VRIITTAVDK RVNDLFRIIP GIGNFGDRYF GTDAVPDGSD 540
EEEVAYTG 548 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016773; F:phosphotransferase activity, alcohol group as acceptor; IEA:InterPro.
 GO:0004849; F:uridine kinase activity; IEA:EC.
 GO:0044211; P:CTP salvage; IEA:UniProtKB-UniPathway.
 GO:0044206; P:UMP salvage; IEA:UniProtKB-UniPathway. 
Interpro
 IPR027417; P-loop_NTPase.
 IPR006083; PRK/URK.
 IPR000764; Uridine_kinase. 
Pfam
 PF00485; PRK 
SMART
  
PROSITE
  
PRINTS
 PR00988; URIDINKINASE.