CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031275
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin carboxyl-terminal hydrolase 16 
Protein Synonyms/Alias
 cDNA FLJ57202, highly similar to Ubiquitin carboxyl-terminal hydrolase 16 (EC 3.1.2.15) 
Gene Name
 USP16 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
55KDNDSYIKERSDIPSubiquitination[1]
66DIPSGTSKHLQKKAKubiquitination[2]
135SQEGVMHKEYCVNQKubiquitination[2, 3]
142KEYCVNQKDLNGQAKubiquitination[3]
149KDLNGQAKMIESVTDubiquitination[4]
167STEEVDMKNINMDNDubiquitination[4]
393GHYTAYAKARTANSHubiquitination[5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Hydrolase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 452 AA 
Protein Sequence
MRGCSYGLCR RGLLFCVILD DQSGKKSVND KNLKKTVEDE DQDSEEEKDN DSYIKERSDI 60
PSGTSKHLQK KAKKQAKKQA KNQRRQQKIQ GKVLHLNDIC TIDHPEDSEY EAEMSLQGEV 120
NIKSNHISQE GVMHKEYCVN QKDLNGQAKM IESVTDNQKS TEEVDMKNIN MDNDLEVLTS 180
SPTRNLNGAY LTEGSNGEVD ISNGFKNLNL NAALHPDEIN IEILNDSHTP GTKVYEVVNE 240
DPETAFCTLA NREVFNTDEC SIQHCLYQFT RNEKLRDANK LLCEVCTRRQ CNGPKANIKG 300
ERKHVYTNAK KQMLISLAPP VLTLHLKRFQ QAGFNLRKVN KHIKFPEILD LAPFCTLKCK 360
NVAEENTRVL YSLYGVVEHS GTMRSGHYTA YAKARTANSH LSNLVLHGDI PQDFEMESKG 420
QWFHISDTHV QAVPTTKVLN SQAYLLFYER IL 452 
Gene Ontology
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19. 
Pfam
 PF00443; UCH 
SMART
  
PROSITE
 PS00973; UCH_2_2
 PS50235; UCH_2_3 
PRINTS