CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000578
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine--pyruvate aminotransferase, mitochondrial 
Protein Synonyms/Alias
 SPT; Alanine--glyoxylate aminotransferase; AGT 
Gene Name
 Agxt 
Gene Synonyms/Alias
 Agxt1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
38PPPEALSKPLSVPTRacetylation[1, 2]
247SLISFNDKAKYKVYSacetylation[1, 2, 3]
247SLISFNDKAKYKVYSsuccinylation[3]
247SLISFNDKAKYKVYSubiquitination[4]
249ISFNDKAKYKVYSRKacetylation[2]
256KYKVYSRKTKPVSFYacetylation[1]
258KVYSRKTKPVSFYTDubiquitination[4]
322EATAHLHKHLQEMGLacetylation[1, 2]
330HLQEMGLKFFVKDPEacetylation[1, 2, 3, 5]
330HLQEMGLKFFVKDPEsuccinylation[3]
334MGLKFFVKDPEIRLPacetylation[1, 2, 3, 6]
334MGLKFFVKDPEIRLPsuccinylation[3]
334MGLKFFVKDPEIRLPubiquitination[4]
Reference
 [1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [2] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [5] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [6] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199] 
Functional Description
 Dual metabolic roles of gluconeogenesis (in the mitochondria) and glyoxylate detoxification (in the peroxisomes) (By similarity). 
Sequence Annotation
 MOTIF 412 414 Microbody targeting signal (By
 BINDING 382 382 Substrate (By similarity).
 MOD_RES 231 231 N6-(pyridoxal phosphate)lysine (By  
Keyword
 3D-structure; Alternative initiation; Aminotransferase; Complete proteome; Mitochondrion; Peroxisome; Pyridoxal phosphate; Reference proteome; Transferase; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 414 AA 
Protein Sequence
MFRMLAKASV TLGSRAAGWV RTMGSYQLLV PPPEALSKPL SVPTRLLLGP GPSNLAPRVL 60
AAGSLRMIGH MQKEMLQIME EIKQGIQYVF QTRNPLTLVV SGSGHCAMET ALFNLLEPGD 120
SFLTGTNGIW GMRAAEIADR IGARVHQMIK KPGEHYTLQE VEEGLAQHKP VLLFLVHGES 180
STGVVQPLDG FGELCHRYQC LLLVDSVASL GGVPIYMDQQ GIDIMYSSSQ KVLNAPPGIS 240
LISFNDKAKY KVYSRKTKPV SFYTDITYLA KLWGCEGETR VIHHTTPVTS LYCLRESLAL 300
IAEQGLENCW RRHREATAHL HKHLQEMGLK FFVKDPEIRL PTITTVTVPA GYNWRDIVSY 360
VLDHFSIEIS GGLGPTEERV LRIGLLGYNA TTENVDRVAE ALREALQHCP KNKL 414 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; TAS:HGNC.
 GO:0005777; C:peroxisome; TAS:HGNC.
 GO:0008453; F:alanine-glyoxylate transaminase activity; IMP:MGI.
 GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
 GO:0004760; F:serine-pyruvate transaminase activity; IEA:EC.
 GO:0046487; P:glyoxylate metabolic process; IMP:MGI.
 GO:0046724; P:oxalic acid secretion; IMP:MGI. 
Interpro
 IPR000192; Aminotrans_V/Cys_dSase.
 IPR020578; Aminotrans_V_PyrdxlP_BS.
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR015422; PyrdxlP-dep_Trfase_major_sub2.
 IPR024169; SP_NH2Trfase/AEP_transaminase. 
Pfam
 PF00266; Aminotran_5 
SMART
  
PROSITE
 PS00595; AA_TRANSFER_CLASS_5 
PRINTS