CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000674
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 U4/U6.U5 tri-snRNP-associated protein 1 
Protein Synonyms/Alias
 SNU66 homolog; hSnu66; Squamous cell carcinoma antigen recognized by T-cells 1; SART-1; hSART-1; U4/U6.U5 tri-snRNP-associated 110 kDa protein; Hom s 1 
Gene Name
 SART1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
94EPSERRVKREKRDDGsumoylation[1]
129ETNKLRAKLGLKPLEubiquitination[2, 3, 4, 5]
141PLEVNAIKKEAGTKEsumoylation[1, 6]
183EKRLLNQKLGKIKTLubiquitination[7, 8]
188NQKLGKIKTLGEDDPubiquitination[9]
302VDKERAEKNVELRKKubiquitination[8]
524QLRDSGEKVVEIVKKubiquitination[7]
530EKVVEIVKKLESRQRubiquitination[8]
648ALLLCQNKGLLETTVubiquitination[7]
657LLETTVQKVARVKAPubiquitination[7, 8]
684DKMAIDDKYSRREEYubiquitination[7]
699RGFTQDFKEKDGYKPubiquitination[7, 8]
723TGRKLTPKEAFRQLSubiquitination[7, 8]
758DEEALLKKMSSSDTPubiquitination[7]
Reference
 [1] Positively charged amino acids flanking a sumoylation consensus tetramer on the 110kDa tri-snRNP component SART1 enhance sumoylation efficiency.
 Schimmel J, Balog CI, Deelder AM, Drijfhout JW, Hensbergen PJ, Vertegaal AC.
 J Proteomics. 2010 Jun 16;73(8):1523-34. [PMID: 20346425]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Site-specific identification of SUMO-2 targets in cells reveals an inverted SUMOylation motif and a hydrophobic cluster SUMOylation motif.
 Matic I, Schimmel J, Hendriks IA, van Santen MA, van de Rijke F, van Dam H, Gnad F, Mann M, Vertegaal AC.
 Mol Cell. 2010 Aug 27;39(4):641-52. [PMID: 20797634]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [9] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA. 
Sequence Annotation
 MOD_RES 392 392 Phosphothreonine.
 MOD_RES 430 430 Phosphothreonine.
 MOD_RES 448 448 Phosphoserine.
 MOD_RES 521 521 Phosphoserine.
 MOD_RES 591 591 Phosphoserine.
 MOD_RES 596 596 Phosphoserine.
 MOD_RES 598 598 Phosphoserine.
 MOD_RES 695 695 Phosphothreonine.
 MOD_RES 764 764 Phosphothreonine.  
Keyword
 Allergen; Alternative splicing; Coiled coil; Complete proteome; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Spliceosome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 800 AA 
Protein Sequence
MGSSKKHRGE KEAAGTTAAA GTGGATEQPP RHREHKKHKH RSGGSGGSGG ERRKRSRERG 60
GERGSGRRGA EAEARSSTHG RERSQAEPSE RRVKREKRDD GYEAAASSKT SSGDASSLSI 120
EETNKLRAKL GLKPLEVNAI KKEAGTKEEP VTADVINPMA LRQREELREK LAAAKEKRLL 180
NQKLGKIKTL GEDDPWLDDT AAWIERSRQL QKEKDLAEKR AKLLEEMDQE FGVSTLVEEE 240
FGQRRQDLYS ARDLQGLTVE HAIDSFREGE TMILTLKDKG VLQEEEDVLV NVNLVDKERA 300
EKNVELRKKK PDYLPYAEDE SVDDLAQQKP RSILSKYDEE LEGERPHSFR LEQGGTADGL 360
RERELEEIRA KLRLQAQSLS TVGPRLASEY LTPEEMVTFK KTKRRVKKIR KKEKEVVVRA 420
DDLLPLGDQT QDGDFGSRLR GRGRRRVSEV EEEKEPVPQP LPSDDTRVEN MDISDEEEGG 480
APPPGSPQVL EEDEAELELQ KQLEKGRRLR QLQQLQQLRD SGEKVVEIVK KLESRQRGWE 540
EDEDPERKGA IVFNATSEFC RTLGEIPTYG LAGNREEQEE LMDFERDEER SANGGSESDG 600
EENIGWSTVN LDEEKQQQDF SASSTTILDE EPIVNRGLAA ALLLCQNKGL LETTVQKVAR 660
VKAPNKSLPS AVYCIEDKMA IDDKYSRREE YRGFTQDFKE KDGYKPDVKI EYVDETGRKL 720
TPKEAFRQLS HRFHGKGSGK MKTERRMKKL DEEALLKKMS SSDTPLGTVA LLQEKQKAQK 780
TPYIVLSGSG KSMNANTITK 800 
Gene Ontology
 GO:0015030; C:Cajal body; IDA:BHF-UCL.
 GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:UniProtKB.
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0007050; P:cell cycle arrest; NAS:UniProtKB.
 GO:0097193; P:intrinsic apoptotic signaling pathway; NAS:UniProtKB.
 GO:0045585; P:positive regulation of cytotoxic T cell differentiation; IDA:UniProtKB.
 GO:0000387; P:spliceosomal snRNP assembly; TAS:BHF-UCL. 
Interpro
 IPR005011; SART_1. 
Pfam
 PF03343; SART-1 
SMART
  
PROSITE
  
PRINTS