CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001183
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cell division control protein 45 homolog 
Protein Synonyms/Alias
 PORC-PI-1 
Gene Name
 CDC45 
Gene Synonyms/Alias
 CDC45L; CDC45L2; UNQ374/PRO710 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
8MFVSDFRKEFYEVVQubiquitination[1, 2, 3]
237QDKITQMKYVTDVGVubiquitination[1, 2, 3, 4]
301SYTAARFKLWSVHGQubiquitination[3]
309LWSVHGQKRLQEFLAubiquitination[3]
323ADMGLPLKQVKQKFQubiquitination[1, 2, 3, 4, 5, 6]
326GLPLKQVKQKFQAMDubiquitination[3]
328PLKQVKQKFQAMDISubiquitination[2, 3, 7]
337QAMDISLKENLREMIubiquitination[1, 2, 3, 4, 6]
350MIEESANKFGMKDMRubiquitination[1, 3, 6]
354SANKFGMKDMRVQTFubiquitination[2, 3]
411LSRSNLDKLYHGLELubiquitination[1, 3, 4, 6, 7, 8]
420YHGLELAKKQLRATQubiquitination[2, 3, 6]
421HGLELAKKQLRATQQubiquitination[3]
471ASLSLLSKHLLKSFVubiquitination[3]
475LLSKHLLKSFVCSTKubiquitination[3, 4]
482KSFVCSTKNRRCKLLubiquitination[3, 6]
528FFGRAFEKAAESTSSubiquitination[2, 3, 4, 6, 9, 10]
550DLSVIELKAEDRSKFubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [9] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [10] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
 Required for initiation of chromosomal DNA replication. 
Sequence Annotation
 MOD_RES 130 130 Phosphotyrosine.
 MOD_RES 144 144 Phosphoserine.
 MOD_RES 148 148 Phosphoserine.  
Keyword
 Alternative splicing; Cell cycle; Complete proteome; Cytoplasm; DNA replication; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 566 AA 
Protein Sequence
MFVSDFRKEF YEVVQSQRVL LFVASDVDAL CACKILQALF QCDHVQYTLV PVSGWQELET 60
AFLEHKEQFH YFILINCGAN VDLLDILQPD EDTIFFVCDT HRPVNVVNVY NDTQIKLLIK 120
QDDDLEVPAY EDIFRDEEED EEHSGNDSDG SEPSEKRTRL EEEIVEQTMR RRQRREWEAR 180
RRDILFDYEQ YEYHGTSSAM VMFELAWMLS KDLNDMLWWA IVGLTDQWVQ DKITQMKYVT 240
DVGVLQRHVS RHNHRNEDEE NTLSVDCTRI SFEYDLRLVL YQHWSLHDSL CNTSYTAARF 300
KLWSVHGQKR LQEFLADMGL PLKQVKQKFQ AMDISLKENL REMIEESANK FGMKDMRVQT 360
FSIHFGFKHK FLASDVVFAT MSLMESPEKD GSGTDHFIQA LDSLSRSNLD KLYHGLELAK 420
KQLRATQQTI ASCLCTNLVI SQGPFLYCSL MEGTPDVMLF SRPASLSLLS KHLLKSFVCS 480
TKNRRCKLLP LVMAAPLSME HGTVTVVGIP PETDSSDRKN FFGRAFEKAA ESTSSRMLHN 540
HFDLSVIELK AEDRSKFLDA LISLLS 566 
Gene Ontology
 GO:0005813; C:centrosome; IDA:HPA.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0000076; P:DNA replication checkpoint; TAS:ProtInc.
 GO:0006270; P:DNA replication initiation; TAS:ProtInc.
 GO:0006271; P:DNA strand elongation involved in DNA replication; TAS:Reactome.
 GO:0000083; P:regulation of transcription involved in G1/S phase of mitotic cell cycle; TAS:Reactome. 
Interpro
 IPR003874; CDC45. 
Pfam
 PF02724; CDC45 
SMART
  
PROSITE
  
PRINTS