CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024122
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Interleukin enhancer-binding factor 3 
Protein Synonyms/Alias
  
Gene Name
 Ilf3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
113MRVGLVAKGLLLKGDubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N- methyltransferase 1 activity. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA (By similarity). The phosphorylated form at Thr-188 and Thr-315, in concert with EIF2AK2/PKR can inhibit vesicular stomatitis virus (VSV) replication. 
Sequence Annotation
 DOMAIN 94 342 DZF.
 DOMAIN 398 467 DRBM 1.
 DOMAIN 524 590 DRBM 2.
 REGION 609 898 Interaction with PRMT1 (By similarity).
 MOTIF 371 389 Bipartite nuclear localization signal
 MOD_RES 100 100 N6-acetyllysine (By similarity).
 MOD_RES 188 188 Phosphothreonine; by PKR.
 MOD_RES 190 190 Phosphoserine (By similarity).
 MOD_RES 315 315 Phosphothreonine; by PKR.
 MOD_RES 382 382 Phosphoserine (By similarity).
 MOD_RES 384 384 Phosphoserine (By similarity).
 MOD_RES 460 460 N6-acetyllysine (By similarity).
 MOD_RES 476 476 Phosphoserine (By similarity).
 MOD_RES 482 482 Phosphoserine (By similarity).
 MOD_RES 592 592 Phosphothreonine (By similarity).
 MOD_RES 609 609 Omega-N-methylated arginine (By
 MOD_RES 794 794 Phosphoserine (By similarity).
 MOD_RES 814 814 Phosphoserine (By similarity).  
Keyword
 3D-structure; Acetylation; Alternative splicing; Antiviral defense; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Methylation; Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 898 AA 
Protein Sequence
MALYHHHFIT RRRRRPMRIF VNDDRHVMAK HSSVYPTQEE LEAVQNMVSH TERALKAVSD 60
WIDEQEKGNS ELSEAENMDT PPDDESKEGA GEQKAEHMTR TLRGVMRVGL VAKGLLLKGD 120
LDLELVLLCK EKPTTALLDK VADNLAIQLT TVTEDKYEIL QSVDDAAIVI KNTKEPPLSL 180
TIHLTSPVVR EEMEKVLAGE TLSVNDPPDV LDRQKCLAAL ASLRHAKWFQ ARANGLKSCV 240
IVIRVLRDLC TRVPTWGPLR GWPLELLCEK SIGTANRPMG AGEALRRVLE CLASGIVMPD 300
GSGIYDPCEK EATDAIGHLD RQQREDITQS AQHALRLAAF GQLHKVLGMD PLPSKMPKKP 360
KNENPVDYTV QIPPSTTYAI TPMKRPMEED GEEKSPSKKK KKIQKKEEKA DPPQAMNALM 420
RLNQLKPGLQ YKLISQTGPV HAPIFTMSVE VDGSNFEASG PSKKTAKLHV AVKVLQDMGL 480
PTGAEGRDSS KGEDSAEESD GKPAIVAPPP VVEAVSNPSS VFPSDATTEQ GPILTKHGKN 540
PVMELNEKRR GLKYELISET GGSHDKRFVM EVEVDGQKFQ GAGSNKKVAK AYAALAALEK 600
LFPDTPLALE ANKKKRTPVP VRGGPKFAAK PHNPGFGMGG PMHNEVPPPP NIRGRGRGGN 660
IRGRGRGRGF GGANHGGGYM NAGAGYGSYG YSSNSATAGY SQFYSNGGHS GNAGGGGSGG 720
GGGSSSYSSY YQGDSYNSPV PPKHAGKKPL HGGQQKASYS SGYQSHQGQQ QPYNQSQYSS 780
YGTPQGKQKG YGHGQGSYSS YSNSYNSPGG GGGSDYSYDS KFNYSGSGGR SGGNSYGSSG 840
SSSYNTGSHG GYGTGSGGSS SYQGKQGGYS SQSNYSSPGS SQSYSGPASS YQSSQGGYSR 900
NTEHSMNYQY R 911 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0030529; C:ribonucleoprotein complex; ISS:UniProtKB.
 GO:0003677; F:DNA binding; TAS:MGI.
 GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
 GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
 GO:0017148; P:negative regulation of translation; IMP:UniProtKB.
 GO:0045071; P:negative regulation of viral genome replication; IMP:UniProtKB.
 GO:0006479; P:protein methylation; ISA:MGI.
 GO:0006468; P:protein phosphorylation; IDA:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001159; Ds-RNA-bd.
 IPR014720; dsRNA-bd-like_dom.
 IPR006561; DZF. 
Pfam
 PF00035; dsrm
 PF07528; DZF 
SMART
 SM00358; DSRM
 SM00572; DZF 
PROSITE
 PS50137; DS_RBD 
PRINTS