CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019621
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TP53-regulating kinase 
Protein Synonyms/Alias
 Nori-2; p53-related protein kinase 
Gene Name
 TP53RK 
Gene Synonyms/Alias
 C20orf64; PRPK 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
40LSGLELVKQGAEARVubiquitination[1, 2, 3]
134QSTMETEKTPQGLSNubiquitination[1, 4]
144QGLSNLAKTIGQVLAubiquitination[3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation. 
Sequence Annotation
 DOMAIN 33 253 Protein kinase.
 NP_BIND 39 47 ATP (By similarity).
 MOTIF 78 95 Nuclear localization signal (Potential).
 ACT_SITE 162 162 Proton acceptor (By similarity).
 BINDING 60 60 ATP (By similarity).
 MOD_RES 34 34 Phosphoserine.
 MOD_RES 135 135 Phosphothreonine.  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 253 AA 
Protein Sequence
MAAARATTPA DGEEPAPEAE ALAAARERSS RFLSGLELVK QGAEARVFRG RFQGRAAVIK 60
HRFPKGYRHP ALEARLGRRR TVQEARALLR CRRAGISAPV VFFVDYASNC LYMEEIEGSV 120
TVRDYIQSTM ETEKTPQGLS NLAKTIGQVL ARMHDEDLIH GDLTTSNMLL KPPLEQLNIV 180
LIDFGLSFIS ALPEDKGVDL YVLEKAFLST HPNTETVFEA FLKSYSTSSK KARPVLKKLD 240
EVRLRGRKRS MVG 253 
Gene Ontology
 GO:0016020; C:membrane; IEA:InterPro.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0002039; F:p53 binding; IDA:HGNC.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0004713; F:protein tyrosine kinase activity; IEA:InterPro.
 GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:InterPro. 
Interpro
 IPR022495; Kae1-assoc_kinase_Bud32.
 IPR011009; Kinase-like_dom.
 IPR010440; LipoPS_kinase.
 IPR000719; Prot_kinase_cat_dom.
 IPR008266; Tyr_kinase_AS. 
Pfam
 PF06293; Kdo 
SMART
  
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00109; PROTEIN_KINASE_TYR 
PRINTS