CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019217
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WD repeat-containing protein 34 
Protein Synonyms/Alias
  
Gene Name
 WDR34 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
361TEGGFPLKCSLAAGEubiquitination[1, 2, 3, 4]
467VQLFDLQKSSQKPTVubiquitination[3, 4]
471DLQKSSQKPTVLIKQubiquitination[3, 4, 5]
477QKPTVLIKQTQDESPubiquitination[3]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
 Acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways. Inhibits the MAP3K7-induced NF-kappa- B activation pathway. Inhibits MAP3K7 phosphorylation at 'Thr-184' and 'Thr-187' upon Il-1 beta stimulation. 
Sequence Annotation
 REPEAT 215 255 WD 1.
 REPEAT 264 308 WD 2.
 REPEAT 390 430 WD 3.
 REPEAT 433 473 WD 4.
 REPEAT 480 520 WD 5.  
Keyword
 Complete proteome; Cytoplasm; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 536 AA 
Protein Sequence
MATRAQPGPL SQAGSAGVAA LATVGVASGP GPGRPGPLQD ETLGVASVPS QWRAVQGIRW 60
ETKSCQTASI ATASASAQAR NHVDAQVQTE APVPVSVQPP SQYDIPRLAA FLRRVEAMVI 120
RELNKNWQSH AFDGFEVNWT EQQQMVSCLY TLGYPPAQAQ GLHVTSISWN STGSVVACAY 180
GRLDHGDWST LKSFVCAWNL DRRDLRPQQP SAVVEVPSAV LCLAFHPTQP SHVAGGLYSG 240
EVLVWDLSRL EDPLLWRTGL TDDTHTDPVS QVVWLPEPGH SHRFQVLSVA TDGKVLLWQG 300
IGVGQLQLTE GFALVMQQLP RSTKLKKHPR GETEVGATAV AFSSFDPRLF ILGTEGGFPL 360
KCSLAAGEAA LTRMPSSVPL RAPAQFTFSP HGGPIYSVSC SPFHRNLFLS AGTDGHVHLY 420
SMLQAPPLTS LQLSLKYLFA VRWSPVRPLV FAAASGKGDV QLFDLQKSSQ KPTVLIKQTQ 480
DESPVYCLEF NSQQTQLLAA GDAQGTVKVW QLSTEFTEQG PREAEDLDCL AAEVAA 536 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. 
Interpro
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS