UniProt Accession

 CYB5B_MOUSE; Q9CQX2; Q9D1M6; Q9D8R3 

Genbank Protein ID

 AK003333; AK003815; AK007780; AK019263; AK052810; AK088970; BC054749; BC058812; BC062980 

Genbank Nucleotide ID

 BAB22721.1; BAB23012.1; BAB25251.1; BAB31635.1; BAC35156.1; BAC40677.1; AAH54749.1; AAH58812.1; AAH62980.1 

Protein Name

 Cytochrome b5 type B 

Protein Synonyms/Alias

 Cytochrome b5 outer mitochondrial membrane isoform 

Gene Name

 Cyb5b 

Gene Synonyms/Alias

 Cyb5m 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
12	EASGSGEKVEGSEPS	acetylation	[1, 2]
30	YRLEEVAKRNSAEET	ubiquitination	[3]
88	PDAREMLKQYYIGDV	acetylation	[1]
88	PDAREMLKQYYIGDV	ubiquitination	[3]
101	DVHPSDLKPKGDDKD	acetylation	[1, 4]

Reference

 [1] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases (By similarity). 

Sequence Annotation

 DOMAIN 20 96 Cytochrome b5 heme-binding.
 METAL 55 55 Iron (heme axial ligand) (By similarity).
 METAL 79 79 Iron (heme axial ligand) (By similarity).
 MOD_RES 30 30 N6-acetyllysine (By similarity).
 MOD_RES 33 33 Phosphoserine.  

Keyword

 Acetylation; Complete proteome; Electron transport; Heme; Iron; Membrane; Metal-binding; Mitochondrion; Mitochondrion outer membrane; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 146 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
 GO:0008047; F:enzyme activator activity; IEA:Compara.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW. 

Interpro

 IPR001199; Cyt_B5-like_heme/steroid-bd.
 IPR018506; Cyt_B5_heme-BS. 

Pfam

 PF00173; Cyt-b5 

SMART

  

PROSITE

 PS00191; CYTOCHROME_B5_1
 PS50255; CYTOCHROME_B5_2 

PRINTS

 PR00363; CYTOCHROMEB5.