CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032134
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin conjugation factor E4 A 
Protein Synonyms/Alias
 cDNA FLJ51394, highly similar to Ubiquitin conjugation factor E4 A 
Gene Name
 UBE4A 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
238DTYRESIKDLADYASubiquitination[1, 2]
246DLADYASKNLEAMNPubiquitination[1, 3, 4]
282IQYLSKIKIQQIEKDubiquitination[3]
304TPEARREKEAGLQMFubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 538 AA 
Protein Sequence
MVKINQNLHR LQVAWRDAQQ SSSPAADNLR EQFERLMTIY LSTKTAMTEP QMLQNCLNLQ 60
VSMAVLLVQL AIGNEGSQPI ELTFPLPDGY SSLAYVPEFF ADNLGDFLIF LRRFADDILE 120
TSADSLEHVL HFITIFTGSI ERMKNPHLRA KLAEVLEAVM PHLDQTPNPL VSSVFHRKRV 180
FCNFQYAPQL AEALIKVFVD IEFTGDPHQF EQKFNYRRPM YPILRYMWGT DTYRESIKDL 240
ADYASKNLEA MNPPLFLRFL NLLMNDAIFL LDEAIQYLSK IKIQQIEKDR GEWDSLTPEA 300
RREKEAGLQM FGQLARFHNI MSNETIGTLA FLTSEIKSLF VHPFLAERII SMLNYFLQHL 360
VGPKMGALKV KDFSEFDFKP QQLVSDICTI YLNLGDEENF CATVPKDGRS YSPTLFAQTV 420
RVLKKINKPG NMIMAFSNLA ERIKSLADLQ QQEEETYADA CDEFLDPIMS TLMCDPVVLP 480
SSRVTVDRST IARHLLSDQT DPFNRSPLTM DQIRPNTELK EKIQRWLAER KQQKEQLE 538 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0000151; C:ubiquitin ligase complex; IEA:InterPro.
 GO:0034450; F:ubiquitin-ubiquitin ligase activity; IEA:Compara.
 GO:0000209; P:protein polyubiquitination; IEA:Compara.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR019474; Ub_conjug_fac_E4_core.
 IPR003613; Ubox_domain.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF04564; U-box
 PF10408; Ufd2P_core 
SMART
 SM00504; Ubox 
PROSITE
  
PRINTS