UniProt Accession

 GGH_HUMAN; Q92820 

Genbank Protein ID

 U55206; AF147083; AF147081; AF147082; BC025025 

Genbank Nucleotide ID

 AAC05579.1; AAF03360.1; AAF03360.1; AAF03360.1; AAH25025.1 

Protein Name

 Gamma-glutamyl hydrolase 

Protein Synonyms/Alias

 Conjugase; GH; Gamma-Glu-X carboxypeptidase 

Gene Name

 GGH 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
81	VRLDLTEKDYEILFK	ubiquitination	[1]
212	TMNEKLKKFFNVLTT	ubiquitination	[2, 3]
235	ISTMEGYKYPVYGVQ	ubiquitination	[2, 3]
253	EKAPYEWKNLDGISH	ubiquitination	[2, 3]
287	RKNNHHFKSESEEEK	ubiquitination	[2, 3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha- glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. 

Sequence Annotation

 DOMAIN 25 318 Gamma-glutamyl hydrolase.
 ACT_SITE 134 134 Nucleophile.
 ACT_SITE 244 244 Proton donor.
 CARBOHYD 116 116 N-linked (GlcNAc...) (Potential).
 CARBOHYD 163 163 N-linked (GlcNAc...).
 CARBOHYD 203 203 N-linked (GlcNAc...).
 CARBOHYD 307 307 N-linked (GlcNAc...); partial.  

Keyword

 3D-structure; Complete proteome; Glycoprotein; Hydrolase; Lysosome; Polymorphism; Reference proteome; Secreted; Signal. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 318 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
 GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0034722; F:gamma-glutamyl-peptidase activity; IDA:UniProtKB.
 GO:0006541; P:glutamine metabolic process; IEA:InterPro.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0045471; P:response to ethanol; IEA:Compara.
 GO:0032868; P:response to insulin stimulus; IEA:Compara.
 GO:0010043; P:response to zinc ion; IEA:Compara. 

Interpro

 IPR015527; Pept_C26_g-glut_hydrolase.
 IPR011697; Peptidase_C26. 

Pfam

 PF07722; Peptidase_C26 

SMART

  

PROSITE

 PS51275; PEPTIDASE_C26_GGH 

PRINTS