CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006180
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear protein STH1/NPS1 
Protein Synonyms/Alias
 ATP-dependent helicase STH1; Chromatin structure-remodeling complex protein STH1; SNF2 homolog 
Gene Name
 STH1 
Gene Synonyms/Alias
 NPS1; YIL126W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
156IEVDYSEKKPIKISAacetylation[1]
183SKFSNATKTALGDPDubiquitination[2]
842MRLDGSTKTEERTEMubiquitination[2]
1179SQENGGAKVEEEVKSacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Catalytic component of the chromatin structure- remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is the essential ATPase of the complex. It is a DNA translocase capable of nucleosome remodeling. Required for full expression of early meiotic genes. Essential for mitotic growth and repression of CHA1 expression. Also involved in G2 phase control. 
Sequence Annotation
 DOMAIN 307 383 HSA.
 DOMAIN 482 647 Helicase ATP-binding.
 DOMAIN 795 956 Helicase C-terminal.
 DOMAIN 1270 1340 Bromo.
 NP_BIND 495 502 ATP (Probable).
 MOTIF 597 600 DEGH box.
 MOD_RES 38 38 Phosphoserine.  
Keyword
 ATP-binding; Bromodomain; Cell cycle; Chromatin regulator; Complete proteome; Direct protein sequencing; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1359 AA 
Protein Sequence
MLQEQSELMS TVMNNTPTTV AALAAVAAAS ETNGKLGSEE QPEITIPKPR SSAQLEQLLY 60
RYRAIQNHPK ENKLEIKAIE DTFRNISRDQ DIYETKLDTL RKSIDKGFQY DEDLLNKHLV 120
ALQLLEKDTD VPDYFLDLPD TKNDNTTAIE VDYSEKKPIK ISADFNAKAK SLGLESKFSN 180
ATKTALGDPD TEIRISARIS NRINELERLP ANLGTYSLDD CLEFITKDDL SSRMDTFKIK 240
ALVELKSLKL LTKQKSIRQK LINNVASQAH HNIPYLRDSP FTAAAQRSVQ IRSKVIVPQT 300
VRLAEELERQ QLLEKRKKER NLHLQKINSI IDFIKERQSE QWSRQERCFQ FGRLGASLHN 360
QMEKDEQKRI ERTAKQRLAA LKSNDEEAYL KLLDQTKDTR ITQLLRQTNS FLDSLSEAVR 420
AQQNEAKILH GEEVQPITDE EREKTDYYEV AHRIKEKIDK QPSILVGGTL KEYQLRGLEW 480
MVSLYNNHLN GILADEMGLG KTIQSISLIT YLYEVKKDIG PFLVIVPLST ITNWTLEFEK 540
WAPSLNTIIY KGTPNQRHSL QHQIRVGNFD VLLTTYEYII KDKSLLSKHD WAHMIIDEGH 600
RMKNAQSKLS FTISHYYRTR NRLILTGTPL QNNLPELWAL LNFVLPKIFN SAKTFEDWFN 660
TPFANTGTQE KLELTEEETL LIIRRLHKVL RPFLLRRLKK EVEKDLPDKV EKVIKCKLSG 720
LQQQLYQQML KHNALFVGAG TEGATKGGIK GLNNKIMQLR KICNHPFVFD EVEGVVNPSR 780
GNSDLLFRVA GKFELLDRVL PKFKASGHRV LMFFQMTQVM DIMEDFLRMK DLKYMRLDGS 840
TKTEERTEML NAFNAPDSDY FCFLLSTRAG GLGLNLQTAD TVIIFDTDWN PHQDLQAQDR 900
AHRIGQKNEV RILRLITTDS VEEVILERAM QKLDIDGKVI QAGKFDNKST AEEQEAFLRR 960
LIESETNRDD DDKAELDDDE LNDTLARSAD EKILFDKIDK ERMNQERADA KAQGLRVPPP 1020
RLIQLDELPK VFREDIEEHF KKEDSEPLGR IRQKKRVYYD DGLTEEQFLE AVEDDNMSLE 1080
DAIKKRREAR ERRRLRQNGT KENEIETLEN TPEASETSLI ENNSFTAAVD EETNADKETT 1140
ASRSKRRSSR KKRTISIVTA EDKENTQEES TSQENGGAKV EEEVKSSSVE IINGSESKKK 1200
KPKLTVKIKL NKTTVLENND GKRAEEKPES KSPAKKTAAK KTKTKSKSLG IFPTVEKLVE 1260
EMREQLDEVD SHPRTSIFEK LPSKRDYPDY FKVIEKPMAI DIILKNCKNG TYKTLEEVRQ 1320
ALQTMFENAR FYNEEGSWVY VDADKLNEFT DEWFKEHSS 1359 
Gene Ontology
 GO:0000775; C:chromosome, centromeric region; IDA:UniProtKB.
 GO:0016586; C:RSC complex; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0015616; F:DNA translocase activity; IDA:SGD.
 GO:0004386; F:helicase activity; IDA:UniProtKB.
 GO:0070577; F:histone acetyl-lysine binding; IDA:SGD.
 GO:0043044; P:ATP-dependent chromatin remodeling; IDA:UniProtKB.
 GO:0031055; P:chromatin remodeling at centromere; IMP:SGD.
 GO:0007059; P:chromosome segregation; IGI:SGD.
 GO:0007010; P:cytoskeleton organization; IGI:UniProtKB.
 GO:0006302; P:double-strand break repair; IMP:SGD.
 GO:0000086; P:G2/M transition of mitotic cell cycle; IMP:UniProtKB.
 GO:0007126; P:meiosis; IMP:UniProtKB.
 GO:0006337; P:nucleosome disassembly; IDA:SGD.
 GO:0016584; P:nucleosome positioning; IMP:SGD.
 GO:0006355; P:regulation of transcription, DNA-dependent; IMP:UniProtKB.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; IDA:SGD. 
Interpro
 IPR001487; Bromodomain.
 IPR018359; Bromodomain_CS.
 IPR014012; Helicase/SANT-assoc_DNA-bd.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000330; SNF2_N. 
Pfam
 PF00439; Bromodomain
 PF00271; Helicase_C
 PF00176; SNF2_N 
SMART
 SM00297; BROMO
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS00633; BROMODOMAIN_1
 PS50014; BROMODOMAIN_2
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51204; HSA 
PRINTS
 PR00503; BROMODOMAIN.