UniProt Accession

 RSMA_ECOLI; P06992; Q2MCG8 

Genbank Protein ID

 M11054; X04711; U00096; AP009048; M68521; X06536 

Genbank Nucleotide ID

 AAA24049.1; CAA28417.1; AAC73162.1; BAE76038.1; AAA24306.1; CAA29786.1 

Protein Name

 Ribosomal RNA small subunit methyltransferase A 

Protein Synonyms/Alias

 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; High level kasugamycin resistance protein KsgA; Kasugamycin dimethyltransferase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 

Gene Name

 rsmA 

Gene Synonyms/Alias

 ksgA; b0051; JW0050 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
205	ATMPHPVKDVRVLSR	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits. Has also a DNA glycosylase/AP lyase activity that removes C mispaired with oxidized T from DNA, and may play a role in protection of DNA against oxidative stress. 

Sequence Annotation

 BINDING 18 18 S-adenosyl-L-methionine; via carbonyl
 BINDING 20 20 S-adenosyl-L-methionine; via amide
 BINDING 45 45 S-adenosyl-L-methionine; via carbonyl
 BINDING 66 66 S-adenosyl-L-methionine (By similarity).
 BINDING 91 91 S-adenosyl-L-methionine (By similarity).
 BINDING 113 113 S-adenosyl-L-methionine (By similarity).  

Keyword

 3D-structure; Antibiotic resistance; Complete proteome; Cytoplasm; Direct protein sequencing; Methyltransferase; Reference proteome; RNA-binding; rRNA processing; S-adenosyl-L-methionine; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 273 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0052908; F:16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity; IEA:EC.
 GO:0003690; F:double-stranded DNA binding; IDA:EcoCyc.
 GO:0003729; F:mRNA binding; IDA:EcoCyc.
 GO:0000179; F:rRNA (adenine-N6,N6-)-dimethyltransferase activity; IDA:EcoCyc.
 GO:0019843; F:rRNA binding; IDA:EcoCyc.
 GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
 GO:0000028; P:ribosomal small subunit assembly; IMP:EcoCyc.
 GO:0070475; P:rRNA base methylation; IMP:EcoCyc. 

Interpro

 IPR023165; rRNA_Ade_diMease-like.
 IPR020596; rRNA_Ade_Mease_Trfase_CS.
 IPR001737; rRNA_Ade_methylase_transferase.
 IPR020598; rRNA_Ade_methylase_Trfase_N.
 IPR011530; rRNA_adenine_dimethylase. 

Pfam

 PF00398; RrnaAD 

SMART

 SM00650; rADc 

PROSITE

 PS01131; RRNA_A_DIMETH 

PRINTS