CPLM-011920

Genbank Nucleotide ID

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1

Protein Synonyms/Alias

BRG1-associated factor 47; BAF47; Integrase interactor 1 protein; SNF5 homolog; hSNF5

Homo sapiens (Human)

Position	Peptide	Type	References
8	MMMMALSKTFGQKPV	ubiquitination	[1]
13	LSKTFGQKPVKFQLE	ubiquitination	[1]
16	TFGQKPVKFQLEDDG	ubiquitination	[1]
77	KKTKPNTKDHGYTTL	ubiquitination	[1]
92	ATSVTLLKASEVEEI	ubiquitination	[2]
106	ILDGNDEKYKAVSIS	ubiquitination	[1, 2]
108	DGNDEKYKAVSISTE	ubiquitination	[1]
199	DMEIDGQKLRDAFTW	ubiquitination	[1, 2]
292	EKENSPEKFALKLCS	ubiquitination	[3, 4]
324	GQLSWHQKTYAFSEN	ubiquitination	[1]
364	TDAEMEKKIRDQDRN	ubiquitination	[1]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Core component of the BAF (hSWI/SNF) complex. This ATP- dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors- specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand- bound VDR-mediated transrepression of the CYP27B1 gene.

REPEAT 186 245 1.
REPEAT 259 319 2.
REGION 106 183 DNA-binding (Potential).
REGION 183 243 HIV-1 integrase-binding.
REGION 186 319 2 X approximate tandem repeats.
REGION 186 245 MYC-binding.
REGION 304 318 Interaction with PPP1R15A.
MOD_RES 129 129 Phosphoserine.

Activator; Alternative splicing; Cell cycle; Chromatin regulator; Complete proteome; Disease mutation; Host-virus interaction; Mental retardation; Neurogenesis; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation; Tumor suppressor.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0071565; C:nBAF complex; ISS:UniProtKB.
GO:0071564; C:npBAF complex; ISS:UniProtKB.
GO:0000228; C:nuclear chromosome; IEA:InterPro.
GO:0005730; C:nucleolus; IDA:HPA.
GO:0005654; C:nucleoplasm; TAS:ProtInc.
GO:0016514; C:SWI/SNF complex; IDA:UniProtKB.
GO:0001741; C:XY body; IEA:Compara.
GO:0003713; F:transcription coactivator activity; IMP:BHF-UCL.
GO:0043044; P:ATP-dependent chromatin remodeling; IBA:RefGenome.
GO:0001835; P:blastocyst hatching; IEA:Compara.
GO:0030154; P:cell differentiation; IBA:RefGenome.
GO:0015074; P:DNA integration; TAS:ProtInc.
GO:0006281; P:DNA repair; IBA:RefGenome.
GO:0044772; P:mitotic cell cycle phase transition; IBA:RefGenome.
GO:0008285; P:negative regulation of cell proliferation; IBA:RefGenome.
GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
GO:0006337; P:nucleosome disassembly; IDA:BHF-UCL.
GO:0043923; P:positive regulation by host of viral transcription; IMP:BHF-UCL.
GO:0051091; P:positive regulation of sequence-specific DNA binding transcription factor activity; IDA:BHF-UCL.
GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:BHF-UCL.
GO:0045090; P:retroviral genome replication; IDA:MGI.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW.

IPR006939; SNF5.
IPR017393; SWI_SNF_chromatin_remodel_cplx.