UniProt Accession

 PPCEL_HUMAN; Q4J6C6; A7E2X6; D6W5A3; O43163; Q4J6C3; Q4J6C4; Q4ZG39; Q6ZMW7; Q96DW7 

Genbank Protein ID

 DQ023503; DQ023504; DQ023505; DQ023506; DQ023507; AB007896; AK131463; AC013717; CH471053; CH471053; CH471053; BC013193; BC151236 

Genbank Nucleotide ID

 AAY89634.1; AAY89635.1; AAY89636.1; AAY89637.1; AAY89638.1; BAA23709.1; BAD18608.1; AAX88956.1; EAX00275.1; EAX00276.1; EAX00277.1; AAH13193.1; AAI51237.1 

Protein Name

 Prolyl endopeptidase-like 

Protein Synonyms/Alias

 Prolylendopeptidase-like 

Gene Name

 PREPL 

Gene Synonyms/Alias

 KIAA0436 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
95	ENMDAFEKVRTKLET	ubiquitination	[1, 2]
152	LFNLEELKLDQPFID	ubiquitination	[3]
173	DEKYVAAKIRTEDSE	ubiquitination	[2]
426	TYKFAEGKLFEETGH	ubiquitination	[4, 5, 6]
439	GHEDPITKTSRVLRL	ubiquitination	[4, 5, 6, 7]
661	VSYTEKLKEAIAEHA	ubiquitination	[2]

Reference

 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Probable serine peptidase whose precise substrate specificity remains unclear. Does not cleave peptides after a arginine or lysine residue. 

Sequence Annotation

 ACT_SITE 559 559 Charge relay system (By similarity).
 ACT_SITE 645 645 Charge relay system (By similarity).
 ACT_SITE 690 690 Charge relay system.  

Keyword

 Alternative splicing; Complete proteome; Cytoplasm; Hydrolase; Protease; Reference proteome; Serine protease. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 727 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 

Interpro

 IPR001375; Peptidase_S9.
 IPR002470; Peptidase_S9A.
 IPR004106; Peptidase_S9A_B_C_N. 

Pfam

 PF00326; Peptidase_S9
 PF02897; Peptidase_S9_N 

SMART

  

PROSITE

 PS00708; PRO_ENDOPEP_SER 

PRINTS

 PR00862; PROLIGOPTASE.