CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004328
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cyclic AMP-dependent transcription factor ATF-2 
Protein Synonyms/Alias
 cAMP-dependent transcription factor ATF-2; Activating transcription factor 2; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; HB16; cAMP response element-binding protein CRE-BP1 
Gene Name
 ATF2 
Gene Synonyms/Alias
 CREB2; CREBP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
357DPDEKRRKFLERNRAacetylation[1]
374SRCRQKRKVWVQSLEacetylation[1]
409RNEVAQLKQLLLAHKubiquitination[2, 3, 4, 5]
Reference
 [1] Multiple roles for acetylation in the interaction of p300 HAT with ATF-2.
 Karanam B, Wang L, Wang D, Liu X, Marmorstein R, Cotter R, Cole PA.
 Biochemistry. 2007 Jul 17;46(28):8207-16. [PMID: 17590016]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'- GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex. 
Sequence Annotation
 DOMAIN 352 415 bZIP.
 ZN_FING 25 49 C2H2-type.
 REGION 354 374 Basic motif (By similarity).
 REGION 380 408 Leucine-zipper (By similarity).
 MOD_RES 69 69 Phosphothreonine; by MAPK11 and MAPK14.
 MOD_RES 71 71 Phosphothreonine; by MAPK1, MAPK3,
 MOD_RES 90 90 Phosphoserine.
 MOD_RES 112 112 Phosphoserine.
 MOD_RES 116 116 Phosphothreonine.
 MOD_RES 328 328 Phosphoserine.  
Keyword
 3D-structure; Activator; Alternative splicing; Complete proteome; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 505 AA 
Protein Sequence
MKFKLHVNSA RQYKDLWNMS DDKPFLCTAP GCGQRFTNED HLAVHKHKHE MTLKFGPARN 60
DSVIVADQTP TPTRFLKNCE EVGLFNELAS PFENEFKKAS EDDIKKMPLD LSPLATPIIR 120
SKIEEPSVVE TTHQDSPLPH PESTTSDEKE VPLAQTAQPT SAIVRPASLQ VPNVLLTSSD 180
SSVIIQQAVP SPTSSTVITQ APSSNRPIVP VPGPFPLLLH LPNGQTMPVA IPASITSSNV 240
HVPAAVPLVR PVTMVPSVPG IPGPSSPQPV QSEAKMRLKA ALTQQHPPVT NGDTVKGHGS 300
GLVRTQSEES RPQSLQQPAT STTETPASPA HTTPQTQSTS GRRRRAANED PDEKRRKFLE 360
RNRAAASRCR QKRKVWVQSL EKKAEDLSSL NGQLQSEVTL LRNEVAQLKQ LLLAHKDCPV 420
TAMQKKSGYH TADKDDSSED ISVPSSPHTE AIQHSSVSTS NGVSSTSKAE AVATSVLTQM 480
ADQSTEPALS QIVMAPSSQS QPSGS 505 
Gene Ontology
 GO:0016020; C:membrane; IEA:Compara.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0035497; F:cAMP response element binding; IDA:BHF-UCL.
 GO:0008140; F:cAMP response element binding protein binding; IDA:BHF-UCL.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IEA:Compara.
 GO:0000980; F:RNA polymerase II distal enhancer sequence-specific DNA binding; IDA:BHF-UCL.
 GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; IC:BHF-UCL.
 GO:0001076; F:RNA polymerase II transcription factor binding transcription factor activity; IC:BHF-UCL.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0060612; P:adipose tissue development; IEA:Compara.
 GO:0045444; P:fat cell differentiation; IEA:Compara.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0002755; P:MyD88-dependent toll-like receptor signaling pathway; TAS:Reactome.
 GO:0003151; P:outflow tract morphogenesis; IEA:Compara.
 GO:0032915; P:positive regulation of transforming growth factor beta2 production; IEA:Compara.
 GO:0051090; P:regulation of sequence-specific DNA binding transcription factor activity; TAS:Reactome.
 GO:0006970; P:response to osmotic stress; IDA:UniProtKB.
 GO:0051403; P:stress-activated MAPK cascade; TAS:Reactome.
 GO:0034166; P:toll-like receptor 10 signaling pathway; TAS:Reactome.
 GO:0034134; P:toll-like receptor 2 signaling pathway; TAS:Reactome.
 GO:0034138; P:toll-like receptor 3 signaling pathway; TAS:Reactome.
 GO:0034142; P:toll-like receptor 4 signaling pathway; TAS:Reactome.
 GO:0034146; P:toll-like receptor 5 signaling pathway; TAS:Reactome.
 GO:0034162; P:toll-like receptor 9 signaling pathway; TAS:Reactome.
 GO:0038123; P:toll-like receptor TLR1:TLR2 signaling pathway; TAS:Reactome.
 GO:0038124; P:toll-like receptor TLR6:TLR2 signaling pathway; TAS:Reactome.
 GO:0035666; P:TRIF-dependent toll-like receptor signaling pathway; TAS:Reactome. 
Interpro
 IPR004827; bZIP.
 IPR016378; TF_cAMP-dep.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
 PF00170; bZIP_1 
SMART
 SM00338; BRLZ
 SM00355; ZnF_C2H2 
PROSITE
 PS50217; BZIP
 PS00036; BZIP_BASIC
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS