CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003882
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Solute carrier family 2, facilitated glucose transporter member 1 
Protein Synonyms/Alias
 Glucose transporter type 1, erythrocyte/brain; GLUT-1; HepG2 glucose transporter 
Gene Name
 SLC2A1 
Gene Synonyms/Alias
 GLUT1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
245THDLQEMKEESRQMMubiquitination[1, 2, 3, 4]
477GGASQSDKTPEELFHubiquitination[1, 2, 3, 4, 5, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses. 
Sequence Annotation
 REGION 279 281 Defines substrate specificity (By
 MOD_RES 234 234 Phosphothreonine (By similarity).
 MOD_RES 478 478 Phosphothreonine.
 MOD_RES 490 490 Phosphoserine.
 CARBOHYD 45 45 N-linked (GlcNAc...).  
Keyword
 3D-structure; Cell membrane; Complete proteome; Direct protein sequencing; Disease mutation; Dystonia; Epilepsy; Glycoprotein; Membrane; Phosphoprotein; Reference proteome; Sugar transport; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 492 AA 
Protein Sequence
MEPSSKKLTG RLMLAVGGAV LGSLQFGYNT GVINAPQKVI EEFYNQTWVH RYGESILPTT 60
LTTLWSLSVA IFSVGGMIGS FSVGLFVNRF GRRNSMLMMN LLAFVSAVLM GFSKLGKSFE 120
MLILGRFIIG VYCGLTTGFV PMYVGEVSPT ALRGALGTLH QLGIVVGILI AQVFGLDSIM 180
GNKDLWPLLL SIIFIPALLQ CIVLPFCPES PRFLLINRNE ENRAKSVLKK LRGTADVTHD 240
LQEMKEESRQ MMREKKVTIL ELFRSPAYRQ PILIAVVLQL SQQLSGINAV FYYSTSIFEK 300
AGVQQPVYAT IGSGIVNTAF TVVSLFVVER AGRRTLHLIG LAGMAGCAIL MTIALALLEQ 360
LPWMSYLSIV AIFGFVAFFE VGPGPIPWFI VAELFSQGPR PAAIAVAGFS NWTSNFIVGM 420
CFQYVEQLCG PYVFIIFTVL LVLFFIFTYF KVPETKGRTF DEIASGFRQG GASQSDKTPE 480
ELFHPLGADS QV 492 
Gene Ontology
 GO:0016323; C:basolateral plasma membrane; IEA:Compara.
 GO:0005901; C:caveola; IEA:Compara.
 GO:0005911; C:cell-cell junction; IEA:Compara.
 GO:0030864; C:cortical actin cytoskeleton; IDA:UniProtKB.
 GO:0001939; C:female pronucleus; IEA:Compara.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0030496; C:midbody; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0055056; F:D-glucose transmembrane transporter activity; IEA:Compara.
 GO:0033300; F:dehydroascorbic acid transporter activity; IEA:Compara.
 GO:0005355; F:glucose transmembrane transporter activity; TAS:ProtInc.
 GO:0043621; F:protein self-association; IDA:UniProtKB.
 GO:0042910; F:xenobiotic transporter activity; IEA:Compara.
 GO:0042149; P:cellular response to glucose starvation; IEA:Compara.
 GO:0006112; P:energy reserve metabolic process; TAS:Reactome.
 GO:0019852; P:L-ascorbic acid metabolic process; TAS:Reactome.
 GO:0006461; P:protein complex assembly; IDA:UniProtKB.
 GO:0050796; P:regulation of insulin secretion; TAS:Reactome.
 GO:0006970; P:response to osmotic stress; IEA:Compara. 
Interpro
 IPR002439; Glu_transpt_1.
 IPR020846; MFS_dom.
 IPR016196; MFS_dom_general_subst_transpt.
 IPR005828; Sub_transporter.
 IPR003663; Sugar/inositol_transpt.
 IPR005829; Sugar_transporter_CS. 
Pfam
 PF00083; Sugar_tr 
SMART
  
PROSITE
 PS50850; MFS
 PS00216; SUGAR_TRANSPORT_1
 PS00217; SUGAR_TRANSPORT_2 
PRINTS
 PR01190; GLUCTRSPORT1.
 PR00171; SUGRTRNSPORT.