CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-042886
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Transcription activator BRG1 
Protein Synonyms/Alias
  
Gene Name
 SMARCA4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
11LRLDGTTKAEDRGMLubiquitination[1]
93TVNSVEEKILAAAKYubiquitination[2, 3]
99EKILAAAKYKLNVDQubiquitination[2]
101ILAAAKYKLNVDQKVubiquitination[2]
117QAGMFDQKSSSHERRubiquitination[2]
196ELPSWIIKDDAEVERubiquitination[1]
233YSDSLTEKQWLKKITubiquitination[2, 3]
332RGRPPAEKLSPNPPNacetylation[4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 Bromodomain; Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 427 AA 
Protein Sequence
FKYLRLDGTT KAEDRGMLLK TFNEPGSEYF IFLLSTRAGG LGLNLQSADT VIIFDSDWNP 60
HQDLQAQDRA HRIGQQNEVR VLRLCTVNSV EEKILAAAKY KLNVDQKVIQ AGMFDQKSSS 120
HERRAFLQAI LEHEEQDEEE DEVPDDETVN QMIARHEEEF DLFMRMDLDR RREEARNPKR 180
KPRLMEEDEL PSWIIKDDAE VERLTCEEEE EKMFGRGSRH RKEVDYSDSL TEKQWLKKIT 240
GKDIHDTASS VARGLQFQRG LQFCTRASKT LKAIEEGTLE EIEEEVRQKK SSRKRKRDSD 300
AGSSTPTTST RSRDKDDESK KQKKRGRPPA EKLSPNPPNL TKKMKKIVDA VIKYKDSSSG 360
RQLSEVFIQL PSRKELPEYY ELIRKPVDFK KIKERIRNHK YRSLNDLEKD VMLLCQNAQT 420
FNLEGSL 427 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004386; F:helicase activity; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro. 
Interpro
 IPR001487; Bromodomain.
 IPR018359; Bromodomain_CS.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00439; Bromodomain
 PF00271; Helicase_C 
SMART
 SM00297; BROMO
 SM00490; HELICc 
PROSITE
 PS00633; BROMODOMAIN_1
 PS50014; BROMODOMAIN_2
 PS51194; HELICASE_CTER 
PRINTS
 PR00503; BROMODOMAIN.