CPLM-003320

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-003320

UniProt Accession

DHPS_ECOLI; P0AC13; P26282; P78110; Q2M935

Genbank Protein ID

X68776; X68777; L06494; L12968; U01376; U18997; U00096; AP009048

Genbank Nucleotide ID

CAA48676.1; CAA48677.1; AAA23804.1; AAA16123.1; AAA97509.1; AAA57978.1; AAC76209.2; BAE77221.1

Protein Name

Dihydropteroate synthase

Protein Synonyms/Alias

DHPS; Dihydropteroate pyrophosphorylase

Gene Name

folP

Gene Synonyms/Alias

dhpS; b3177; JW3144

Created Date

July 27, 2013

Organism

Escherichia coli (strain K12)

NCBI Taxa ID

83333

Lysine Modification

Position	Peptide	Type	References
153	KTMQEAPKYDDVFAE	acetylation	[1]
181	QAGIAKEKLLLDPGF	acetylation	[1]
260	IIRVHDVKETVEAMR	acetylation	[1]

Reference

[1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]

Functional Description

DHPS catalyzes the formation of the immediate precursor of folic acid. It is implicated in resistance to sulfonamide.

Sequence Annotation

DOMAIN 15 267 Pterin-binding.
REGION 62 63 Substrate binding.
METAL 22 22 Magnesium.
BINDING 30 30 Substrate.
BINDING 96 96 Substrate.
BINDING 115 115 Substrate.
BINDING 185 185 Substrate.
BINDING 221 221 Substrate.
BINDING 255 255 Substrate.
BINDING 257 257 Substrate.

Keyword

3D-structure; Antibiotic resistance; Complete proteome; Direct protein sequencing; Folate biosynthesis; Magnesium; Metal-binding; Reference proteome; Transferase.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

282 AA

Protein Sequence

MKLFAQGTSL DLSHPHVMGI LNVTPDSFSD GGTHNSLIDA VKHANLMINA GATIIDVGGE 60
STRPGAAEVS VEEELQRVIP VVEAIAQRFE VWISVDTSKP EVIRESAKVG AHIINDIRSL 120
SEPGALEAAA ETGLPVCLMH MQGNPKTMQE APKYDDVFAE VNRYFIEQIA RCEQAGIAKE 180
KLLLDPGFGF GKNLSHNYSL LARLAEFHHF NLPLLVGMSR KSMIGQLLNV GPSERLSGSL 240
ACAVIAAMQG AHIIRVHDVK ETVEAMRVVE ATLSAKENKR YE 282

Gene Ontology

GO:0005737; C:cytoplasm; IDA:EcoCyc.
GO:0004156; F:dihydropteroate synthase activity; IDA:EcoCyc.
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO:0046656; P:folic acid biosynthetic process; IMP:EcoCyc.
GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
GO:0042493; P:response to drug; IMP:EcoliWiki.
GO:0046654; P:tetrahydrofolate biosynthetic process; IEA:UniProtKB-UniPathway.

Interpro

IPR006390; DHP_synth.
IPR011005; Dihydropteroate_synth-like.
IPR000489; Pterin-binding.

Pfam

PF00809; Pterin_bind

SMART

PROSITE

PS00792; DHPS_1
PS00793; DHPS_2
PS50972; PTERIN_BINDING

PRINTS