CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031131
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Macrophage erythroblast attacher 
Protein Synonyms/Alias
 cDNA FLJ53639, highly similar to Homo sapiens macrophage erythroblast attacher (MAEA), transcript variant 1, mRNA 
Gene Name
 MAEA 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
34VEKLSVLKRKAVESIubiquitination[1]
36KLSVLKRKAVESIQAubiquitination[1, 2]
49QAEDESAKLCKRRIEubiquitination[1, 3]
59KRRIEHLKEHSSDQPubiquitination[1, 2, 3, 4]
73PAAASVWKRKRMDRMubiquitination[1, 2, 3, 4]
96GYYNTAVKLARQSGIubiquitination[1, 3, 4]
138LAWCHDNKSRLRKMKubiquitination[1, 3]
175DAVRHARKHFSQAEGubiquitination[1, 2, 3, 4]
256IKTPQCYKEDGSSKSubiquitination[1, 3]
262YKEDGSSKSPDCPVCubiquitination[1, 3, 4]
275VCSRSLNKLAQPLPMubiquitination[1, 3, 4, 5]
327LSIRQDDKVVCPRTKubiquitination[1]
334KVVCPRTKEVFHFSQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 348 AA 
Protein Sequence
MVVAELEKTL SGCPAVDSVV SLLDGVVEKL SVLKRKAVES IQAEDESAKL CKRRIEHLKE 60
HSSDQPAAAS VWKRKRMDRM MVEHLLRCGY YNTAVKLARQ SGIEDLVNIE MFLTAKEVEE 120
SLERRETATC LAWCHDNKSR LRKMKSCLEF SLRIQEFIEL IRQNKRLDAV RHARKHFSQA 180
EGSQLDEVRQ AMGMLAFPPD THISPYKDLL DPARWRMLIQ QFRYDNYRLH QLGNNSVFTL 240
TLQAGLSAIK TPQCYKEDGS SKSPDCPVCS RSLNKLAQPL PMAHCANSRL VCKISGDVMN 300
ENNPPMMLPN GYVYGYNSLL SIRQDDKVVC PRTKEVFHFS QAEKVYIM 348 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; IEA:Compara.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0007010; P:cytoskeleton organization; IEA:Compara.
 GO:0048822; P:enucleate erythrocyte development; IEA:Compara. 
Interpro
 IPR013144; CRA_dom.
 IPR024964; CTLH/CRA.
 IPR006595; CTLH_C.
 IPR006594; LisH_dimerisation.
 IPR027370; zf-RING_LisH. 
Pfam
 PF10607; CLTH
 PF13445; zf-RING_LisH 
SMART
 SM00757; CRA
 SM00668; CTLH
 SM00667; LisH 
PROSITE
 PS50897; CTLH
 PS50896; LISH 
PRINTS