UniProt Accession

 LDHB_RAT; P42123 

Genbank Protein ID

 U07181; BC059149 

Genbank Nucleotide ID

 AAA50439.1; AAH59149.1 

Protein Name

 L-lactate dehydrogenase B chain 

Protein Synonyms/Alias

 LDH-B; LDH heart subunit; LDH-H 

Gene Name

 Ldhb 

Gene Synonyms/Alias

 Ldh-2; Ldh2 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
7	*MATLKEKLIAPVAD	acetylation	[1]
7	*MATLKEKLIAPVAD	ubiquitination	[2]
58	LVDVLEDKLKGEMMD	acetylation	[1]
58	LVDVLEDKLKGEMMD	methylation	[3]
60	DVLEDKLKGEMMDLQ	acetylation	[1]
60	DVLEDKLKGEMMDLQ	methylation	[3]
82	TPKIVADKDYSVTAN	acetylation	[1]
119	QRNVNVFKFIIPQIV	acetylation	[1]
233	ENWKEVHKMVVDSAY	acetylation	[1]
244	DSAYEVIKLKGYTNW	acetylation	[1]
244	DSAYEVIKLKGYTNW	ubiquitination	[2]
308	LTSVINQKLKDDEVA	acetylation	[1]
308	LTSVINQKLKDDEVA	ubiquitination	[2]
310	SVINQKLKDDEVAQL	acetylation	[1]
310	SVINQKLKDDEVAQL	ubiquitination	[2]
319	DEVAQLRKSADTLWD	acetylation	[1]
329	DTLWDIQKDLKDL**	acetylation	[1]
329	DTLWDIQKDLKDL**	ubiquitination	[2]
332	WDIQKDLKDL*****	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [3] A method for the comprehensive proteomic analysis of membrane proteins.
 Wu CC, MacCoss MJ, Howell KE, Yates JR 3rd.
 Nat Biotechnol. 2003 May;21(5):532-8. [PMID: 12692561] 

Functional Description

  

Sequence Annotation

 NP_BIND 30 58 NAD (By similarity).
 ACT_SITE 194 194 Proton acceptor (By similarity).
 BINDING 100 100 NAD (By similarity).
 BINDING 107 107 Substrate (By similarity).
 BINDING 139 139 NAD or substrate (By similarity).
 BINDING 170 170 Substrate (By similarity).
 BINDING 249 249 Substrate (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 7 7 N6-acetyllysine (By similarity).
 MOD_RES 44 44 Phosphoserine (By similarity).
 MOD_RES 58 58 N6-acetyllysine (By similarity).
 MOD_RES 119 119 N6-acetyllysine (By similarity).
 MOD_RES 240 240 Phosphotyrosine (By similarity).
 MOD_RES 329 329 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; NAD; Oxidoreductase; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 334 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0042802; F:identical protein binding; IDA:RGD.
 GO:0004459; F:L-lactate dehydrogenase activity; IDA:RGD.
 GO:0051287; F:NAD binding; IDA:RGD.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
 GO:0006089; P:lactate metabolic process; IDA:RGD.
 GO:0019674; P:NAD metabolic process; IDA:RGD. 

Interpro

 IPR001557; L-lactate/malate_DH.
 IPR011304; L-lactate_DH.
 IPR018177; L-lactate_DH_AS.
 IPR022383; Lactate/malate_DH_C.
 IPR001236; Lactate/malate_DH_N.
 IPR015955; Lactate_DH/Glyco_Ohase_4_C.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF02866; Ldh_1_C
 PF00056; Ldh_1_N 

SMART

  

PROSITE

 PS00064; L_LDH 

PRINTS

 PR00086; LLDHDRGNASE.