| Tag | Content |
|---|
| CPLM ID | CPLM-023855 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Cysteine protease ATG4B |
Protein Synonyms/Alias | AUT-like 1 cysteine endopeptidase; Autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; hAPG4B |
Gene Name | ATG4B |
Gene Synonyms/Alias | APG4B; AUTL1; KIAA0943 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 153 | NTVAQVLKKLAVFDT | ubiquitination | [1] |
|
Reference | [1] Systematic and quantitative assessment of the ubiquitin-modified proteome. Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP. Mol Cell. 2011 Oct 21;44(2):325-40. [ PMID: 21906983] |
Functional Description | Cysteine protease required for autophagy, which cleaves the C-terminal part of MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Also mediates the lipid deconjugation required for target recycling. |
Sequence Annotation | ACT_SITE 74 74 Nucleophile.
ACT_SITE 278 278 Potential.
ACT_SITE 280 280
MOD_RES 1 1 N-acetylmethionine.
MOD_RES 34 34 Phosphoserine (By similarity).
MOD_RES 383 383 Phosphoserine. |
Keyword | 3D-structure; Acetylation; Alternative splicing; Autophagy; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Phosphoprotein; Polymorphism; Protease; Protein transport; Reference proteome; Thiol protease; Transport; Ubl conjugation pathway. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 393 AA |
Protein Sequence | MDAATLTYDT LRFAEFEDFP ETSEPVWILG RKYSIFTEKD EILSDVASRL WFTYRKNFPA 60
IGGTGPTSDT GWGCMLRCGQ MIFAQALVCR HLGRDWRWTQ RKRQPDSYFS VLNAFIDRKD 120
SYYSIHQIAQ MGVGEGKSIG QWYGPNTVAQ VLKKLAVFDT WSSLAVHIAM DNTVVMEEIR 180
RLCRTSVPCA GATAFPADSD RHCNGFPAGA EVTNRPSPWR PLVLLIPLRL GLTDINEAYV 240
ETLKHCFMMP QSLGVIGGKP NSAHYFIGYV GEELIYLDPH TTQPAVEPTD GCFIPDESFH 300
CQHPPCRMSI AELDPSIAVG FFCKTEDDFN DWCQQVKKLS LLGGALPMFE LVELQPSHLA 360
CPDVLNLSLD SSDVERLERF FDSEDEDFEI LSL 393 |
Gene Ontology | GO:0005737; C:cytoplasm; ISS:UniProtKB. GO:0008234; F:cysteine-type peptidase activity; IDA:UniProtKB. GO:0004175; F:endopeptidase activity; IDA:BHF-UCL. GO:0000045; P:autophagic vacuole assembly; IGI:UniProtKB. GO:0010508; P:positive regulation of autophagy; IDA:BHF-UCL. GO:0015031; P:protein transport; IEA:UniProtKB-KW. GO:0006508; P:proteolysis; IDA:UniProtKB. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |