CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030799
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 LIM and SH3 domain protein 1 
Protein Synonyms/Alias
 cDNA FLJ51834, highly similar to LIM and SH3 domain protein 1 
Gene Name
 LASP1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
31LLQRVRYKEEFEKNKubiquitination[1]
40EFEKNKGKGFSVVADubiquitination[1, 2, 3, 4]
65QDQISNIKYHEEFEKacetylation[5]
65QDQISNIKYHEEFEKubiquitination[2, 6]
72KYHEEFEKSRMGPSGubiquitination[1]
131AQSYGGYKEPAAPVSacetylation[5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 205 AA 
Protein Sequence
MLPLRDLQDD TEHEELQGLR EEALLQRVRY KEEFEKNKGK GFSVVADTPE LQRIKKTQDQ 60
ISNIKYHEEF EKSRMGPSGG EGMEPERRDS QDGSSYRRPL EQQQPHHIPT SAPVYQQPQQ 120
QPVAQSYGGY KEPAAPVSIQ RSAPGGGGKR YRAVYDYSAA DEDEVSFQDG DTIVNVQQID 180
DGWMYGTVER TGDTGMLPAN YVEAI 205 
Gene Ontology
  
Interpro
 IPR000900; Nebulin_35r-motif.
 IPR001452; SH3_domain. 
Pfam
 PF00880; Nebulin
 PF00018; SH3_1 
SMART
 SM00326; SH3 
PROSITE
 PS51216; NEBULIN
 PS50002; SH3 
PRINTS
 PR00452; SH3DOMAIN.