UniProt Accession

 MPIP3_HUMAN; P30307; D3DQB8; Q96PL3; Q9H168; Q9H2E8; Q9H2E9; Q9H2F1 

Genbank Protein ID

 M34065; AJ304504; AY497474; CH471062; CH471062; BC019089; AF277723; AF277725; AF277726; AF312681 

Genbank Nucleotide ID

 AAA35666.1; CAC19192.1; AAR32098.1; EAW62145.1; EAW62149.1; AAH19089.1; AAG41885.1; AAG41887.1; AAG41888.1; AAL26835.1 

Protein Name

 M-phase inducer phosphatase 3 

Protein Synonyms/Alias

 Dual specificity phosphatase Cdc25C 

Gene Name

 CDC25C 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
52	VPRTPVGKFLGDSAN	ubiquitination	[1]
251	KYFSGQGKLRKGLCL	ubiquitination	[1]
260	RKGLCLKKTVSLCDI	ubiquitination	[1]
298	ALPTVSGKHQDLKYV	ubiquitination	[1]
317	VAALLSGKFQGLIEK	ubiquitination	[1]
360	LFNFFLKKPIVPLDT	ubiquitination	[1]
413	YPELYILKGGYRDFF	ubiquitination	[1, 2]
469	EQIALLVKDMSP***	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 

Functional Description

 Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity. 

Sequence Annotation

 DOMAIN 321 428 Rhodanese.
 REGION 334 379 HIV-1 Vpr binding site.
 ACT_SITE 377 377 By similarity.
 MOD_RES 38 38 Phosphoserine.
 MOD_RES 48 48 Phosphothreonine.
 MOD_RES 57 57 Phosphoserine.
 MOD_RES 61 61 Phosphoserine.
 MOD_RES 64 64 Phosphoserine.
 MOD_RES 67 67 Phosphothreonine.
 MOD_RES 122 122 Phosphoserine; by CDK1 (Probable).
 MOD_RES 129 129 Phosphoserine.
 MOD_RES 130 130 Phosphothreonine; by CDK1 (Probable).
 MOD_RES 168 168 Phosphoserine.
 MOD_RES 191 191 Phosphoserine; by PLK3.
 MOD_RES 198 198 Phosphoserine; by PLK3.
 MOD_RES 214 214 Phosphoserine; by CDK1 (Probable).
 MOD_RES 216 216 Phosphoserine; by CHEK1, CHEK2, BRSK1 and
 MOD_RES 472 472 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Cell cycle; Cell division; Complete proteome; Host-virus interaction; Hydrolase; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Protein phosphatase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 473 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0004725; F:protein tyrosine phosphatase activity; NAS:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0008283; P:cell proliferation; TAS:UniProtKB.
 GO:0006260; P:DNA replication; TAS:Reactome.
 GO:0000086; P:G2/M transition of mitotic cell cycle; IGI:UniProtKB.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0000079; P:regulation of cyclin-dependent protein serine/threonine kinase activity; TAS:UniProtKB.
 GO:0007088; P:regulation of mitosis; TAS:ProtInc.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 

Interpro

 IPR000751; MPI_Phosphatase.
 IPR001763; Rhodanese-like_dom. 

Pfam

 PF06617; M-inducer_phosp
 PF00581; Rhodanese 

SMART

 SM00450; RHOD 

PROSITE

 PS50206; RHODANESE_3 

PRINTS

 PR00716; MPIPHPHTASE.