UniProt Accession

 DGUOK_HUMAN; Q16854; P78532; Q16759; Q4ZG09; Q96BC1 

Genbank Protein ID

 U41668; AC073046; CH471053; BC015757; X97386; U81499 

Genbank Nucleotide ID

 AAC50624.1; AAX88910.1; EAW99709.1; AAH15757.1; CAA66054.1; AAB39858.1 

Protein Name

 Deoxyguanosine kinase, mitochondrial 

Protein Synonyms/Alias

 dGK 

Gene Name

 DGUOK 

Gene Synonyms/Alias

 DGK 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
56	VGKSTFVKLLTKTYP	acetylation	[1, 2]
120	FSFLSRLKVQLEPFP	ubiquitination	[3]
129	QLEPFPEKLLQARKP	ubiquitination	[3, 4, 5]
135	EKLLQARKPVQIFER	ubiquitination	[3, 5]
275	REVNTFVKNL*****	acetylation	[1]
275	REVNTFVKNL*****	ubiquitination	[3, 5, 6]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Required for the phosphorylation of several deoxyribonucleosides and certain nucleoside analogs widely employed as antiviral and chemotherapeutic agents. 

Sequence Annotation

 NP_BIND 45 53 ATP.
 NP_BIND 206 208 ATP.
 ACT_SITE 141 141 Proton acceptor (Potential).
 BINDING 70 70 Substrate (By similarity).
 BINDING 100 100 Substrate.
 BINDING 111 111 Substrate.
 BINDING 118 118 Substrate.
 BINDING 142 142 Substrate.
 BINDING 147 147 Substrate (By similarity).
 BINDING 211 211 Substrate.
 MOD_RES 275 275 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Disease mutation; Kinase; Mitochondrion; Neuropathy; Nucleotide-binding; Reference proteome; Transferase; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 277 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005759; C:mitochondrial matrix; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004138; F:deoxyguanosine kinase activity; IDA:UniProtKB.
 GO:0016773; F:phosphotransferase activity, alcohol group as acceptor; IEA:InterPro.
 GO:0046070; P:dGTP metabolic process; IEA:Compara.
 GO:0008617; P:guanosine metabolic process; TAS:ProtInc.
 GO:0010977; P:negative regulation of neuron projection development; IEA:Compara.
 GO:0006468; P:protein phosphorylation; IEA:Compara.
 GO:0046122; P:purine deoxyribonucleoside metabolic process; IDA:UniProtKB.
 GO:0006144; P:purine nucleobase metabolic process; TAS:Reactome.
 GO:0043101; P:purine-containing compound salvage; TAS:Reactome. 

Interpro

 IPR002624; Deoxynucleoside_kinase.
 IPR027417; P-loop_NTPase. 

Pfam

 PF01712; dNK 

SMART

  

PROSITE

  

PRINTS