CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015277
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 S-phase kinase-associated protein 1 
Protein Synonyms/Alias
 Cyclin-A/CDK2-associated protein p19; S-phase kinase-associated protein 1A; p19A; p19skp1 
Gene Name
 Skp1 
Gene Synonyms/Alias
 Skp1a 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
142IRKTFNIKNDFTEEEubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113
Functional Description
 Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) direct ubiquitination of TTI1 and TELO2 (By similarity). 
Sequence Annotation
 REGION 104 163 Interaction with the F-box domain of F-
 MOD_RES 131 131 Phosphothreonine (By similarity).  
Keyword
 Complete proteome; Phosphoprotein; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 163 AA 
Protein Sequence
MPTIKLQSSD GEIFEVDVEI AKQSVTIKTM LEDLGMDDEG DDDPVPLPNV NAAILKKVIQ 60
WCTHHKDDPP PPEDDENKEK RTDDIPVWDQ EFLKVDQGTL FELILAANYL DIKGLLDVTC 120
KTVANMIKGK TPEEIRKTFN IKNDFTEEEE AQVRKENQWC EEK 163 
Gene Ontology
 GO:0031467; C:Cul7-RING ubiquitin ligase complex; ISS:UniProtKB.
 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0031519; C:PcG protein complex; IEA:Compara.
 GO:0019005; C:SCF ubiquitin ligase complex; ISS:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:Compara.
 GO:0035518; P:histone H2A monoubiquitination; IEA:Compara.
 GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB. 
Interpro
 IPR011333; BTB/POZ_fold.
 IPR016897; E3_ubiquit_lig_SCF_Skp.
 IPR001232; Skp1_comp.
 IPR016072; Skp1_comp_dimer.
 IPR016073; Skp1_comp_POZ. 
Pfam
 PF01466; Skp1
 PF03931; Skp1_POZ 
SMART
 SM00512; Skp1 
PROSITE
  
PRINTS