CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030749
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Anaphase-promoting complex subunit 5 
Protein Synonyms/Alias
 cDNA FLJ55537, highly similar to Anaphase-promoting complex subunit 5 (APC5)(Cyclosome subunit 5) 
Gene Name
 ANAPC5 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
126DRAMNDGKYHLADSLubiquitination[1, 2, 3]
149SIEGVYRKAVVLQAQubiquitination[1, 3]
164NQMSEAHKLLQKLLVubiquitination[1, 4]
271RAMFLVAKCQVASAAubiquitination[2, 3]
284AASYDQPKKAEALEAubiquitination[3]
285ASYDQPKKAEALEAAubiquitination[1, 3]
300IENLNEAKNYFAKVDubiquitination[1, 2, 3, 5, 6, 7]
305EAKNYFAKVDCKERIubiquitination[2, 3, 7]
328RLYHTLGKTQERNRCubiquitination[1, 3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 357 AA 
Protein Sequence
MDALKDSDLL HWKHSLSELI DISIAQKTAI WRLYGRSTMA LQQAQMLLSM NSLEAVNAGV 60
QQNNTESFAV ALCHLAELHA EQGCFAAASE VLKHLKERFP PNSQHAQLWM LCDQKIQFDR 120
AMNDGKYHLA DSLVTGITAL NSIEGVYRKA VVLQAQNQMS EAHKLLQKLL VHCQKLKNTE 180
MVISVLLSVA ELYWRSSSPT IALPMLLQAL ALSKEYRLQY LASETVLNLA FAQLILGIPE 240
QALSLLHMAI EPILADGAIL DKGRAMFLVA KCQVASAASY DQPKKAEALE AAIENLNEAK 300
NYFAKVDCKE RIRDVVYFQA RLYHTLGKTQ ERNRCAMLFR QLHQELPSHG VPLINHL 357 
Gene Ontology
  
Interpro
 IPR011990; TPR-like_helical. 
Pfam
  
SMART
  
PROSITE
  
PRINTS