UniProt Accession

 JAM1_HUMAN; Q9Y624 

Genbank Protein ID

 AF111713; AF207907; AF172398; AL136649; AY358896; BC001533 

Genbank Nucleotide ID

 AAD42050.1; AAF22829.1; AAD48877.1; CAB66584.1; AAQ89255.1; AAH01533.1 

Protein Name

 Junctional adhesion molecule A 

Protein Synonyms/Alias

 JAM-A; Junctional adhesion molecule 1; JAM-1; Platelet F11 receptor; Platelet adhesion molecule 1; PAM-1; CD321 

Gene Name

 F11R 

Gene Synonyms/Alias

 JAM1; JCAM; UNQ264/PRO301 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
97	LPTGITFKSVTREDT	ubiquitination	[1, 2]
178	IVMPTNPKSTRAFSN	ubiquitination	[3]
277	KKGTSSKKVIYSQPS	ubiquitination	[1]
292	ARSEGEFKQTSSFLV	ubiquitination	[2, 3]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. 

Sequence Annotation

 DOMAIN 28 125 Ig-like V-type 1.
 DOMAIN 135 228 Ig-like V-type 2.
 MOD_RES 281 281 Phosphoserine.
 MOD_RES 284 284 Phosphoserine.
 MOD_RES 287 287 Phosphoserine.
 CARBOHYD 185 185 N-linked (GlcNAc...).
 CARBOHYD 191 191 N-linked (GlcNAc...).
 DISULFID 50 109
 DISULFID 153 212  

Keyword

 3D-structure; Cell junction; Cell membrane; Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; Host-virus interaction; Immunoglobulin domain; Membrane; Phosphoprotein; Reference proteome; Repeat; Signal; Tight junction; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 299 AA 

Protein Sequence

Gene Ontology

 GO:0005911; C:cell-cell junction; TAS:ProtInc.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; IDA:LIFEdb.
 GO:0005923; C:tight junction; IEA:UniProtKB-SubCell.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0007155; P:cell adhesion; IEA:Compara.
 GO:0030855; P:epithelial cell differentiation; IEA:Compara.
 GO:0006954; P:inflammatory response; TAS:ProtInc.
 GO:0050900; P:leukocyte migration; TAS:Reactome.
 GO:0070830; P:tight junction assembly; TAS:Reactome.
 GO:0007179; P:transforming growth factor beta receptor signaling pathway; TAS:Reactome.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 

Interpro

 IPR007110; Ig-like_dom.
 IPR013783; Ig-like_fold.
 IPR003598; Ig_sub2.
 IPR013106; Ig_V-set.
 IPR003596; Ig_V-set_subgr. 

Pfam

 PF07686; V-set 

SMART

 SM00408; IGc2
 SM00406; IGv 

PROSITE

 PS50835; IG_LIKE 

PRINTS