CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019685
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ARF3-interacting protein 1 
Protein Synonyms/Alias
  
Gene Name
 AFI1 
Gene Synonyms/Alias
 YOR129C; YOR3296C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
144AENHQDAKNNRYTIVubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Involved in actin patch polarization. Required for maintaining a proper budding pattern in yeast cells. Required for proper polarized localization of the ADP-ribosylation factor ARF3 at the plasma membrane. 
Sequence Annotation
 REGION 25 488 Interaction with active ARF3.  
Keyword
 Coiled coil; Complete proteome; Cytoplasm; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 893 AA 
Protein Sequence
MLRRELNNSI SNRSIENESF PFERPNVSYI ISAEFDNKLG PILKHQYPKD IPGFNQFSHE 60
QRNGNTSVSM NLASLMIPSS IERNPGKQDI TVFTLYYNKF TQNYQLFPVP KDPRFSFNLH 120
HREQSDGSVT NSIYYDAENH QDAKNNRYTI VLEDDELECQ EVQNNQKAID NEPLFFINVA 180
NTVLDTTNDR GAVIKSIAIG TPLKTFFAFK NIIVLVLDLY MKAPTQAAAT DILLDCFNML 240
NSIDLTLIND IHSKSSIQEV LHSIHDESII TKVFLDPDST LKKLFCINGF DTKDKYGNIV 300
TFHDQLIQYH FTRFQPKTLP PFLLKIPLQF NMIRREPIYI ENDYNELVLK FLDKFVPYLL 360
KAGQKVNAWK LVINSTKLSK EDLCAFILSL ANITATYASD PQSYFKGNAA LIFPYMDISL 420
VDGLRAYVAS NSDFVGCFAI IGTANPIFRY QLDIWDYYYD VDEGVFYENN SPEKEKPDTV 480
AEVKIGPNPL RKIFNRPHFS TNAVNESQVN LGQKLFSLLI DEYHDSDTIM SVLRRLNVLQ 540
LENLLDALKR REIPPNIALK DEYIMFYKDF FIFPEFFDYF TLHSIELLSN LDNCLFSLGN 600
TCQLFSTEQI YSQLSQILDI VKELFRMVSV SRTNIEKFLN ACLNYSPFKI LPTAQLHGDN 660
ISRWSFESEV RQGFDNFNSY MGIEKDPHGV IVSAIDLFTQ IYSFDILAFF LTFITKESGQ 720
DLPFTKSLSR RRTYLTRIAQ SSSLRQFLQL STRPNIRILG GNGQGTGNSN YPEFTNASSV 780
ISPKLRASPL LERRASKICY AITKLLYRLE CHPIGMALLK KYLHNQLREA YLESKRHFIS 840
KKGDSTNTSS TIASSSFAGA SVPLSSNESG MLNGLKQINE QQESTLETTQ KED 893 
Gene Ontology
 GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
 GO:0005935; C:cellular bud neck; IDA:SGD.
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IMP:SGD.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IMP:SGD.
 GO:0051666; P:actin cortical patch localization; IMP:SGD.
 GO:0008105; P:asymmetric protein localization; IMP:SGD.
 GO:0000282; P:cellular bud site selection; IMP:SGD. 
Interpro
 IPR012860; Afi1_N. 
Pfam
 PF07792; Afi1 
SMART
  
PROSITE
  
PRINTS