CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-045158
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Ubiquitin-conjugating enzyme E2 O 
Protein Synonyms/Alias
  
Gene Name
 UBE2O 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
35KSIPLSIKNLKRKHKubiquitination[1]
107PGDFVVDKRVQSCPDubiquitination[2, 3, 4]
134IGRTCMVKWFKLRPSubiquitination[1, 3, 4, 5]
324AGKDGPPKSFRELKEubiquitination[3, 4]
330PKSFRELKEAIKILEubiquitination[1, 3, 4]
334RELKEAIKILESLKNubiquitination[1, 3, 4]
340IKILESLKNMTVEQLubiquitination[1, 3, 5, 6]
361SPTVEPEKPTREKKFubiquitination[6]
367EKPTREKKFLDDIKKubiquitination[3]
374KFLDDIKKLQENLKKubiquitination[3]
380KKLQENLKKTLDNVAubiquitination[3]
381KLQENLKKTLDNVAIubiquitination[1, 6]
393VAIVEEEKMEAVPDVubiquitination[6]
505DLFSALIKGPTRTPYubiquitination[1, 3, 6, 7, 8]
669ETHALLEKAQALPNGubiquitination[3]
763YRSFLPEKSGYPDIGubiquitination[3, 4, 6, 8]
778FPLFPLSKGFIKSIRubiquitination[3, 4]
782PLSKGFIKSIRGVLTubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 807 AA 
Protein Sequence
XTDDTSSVTS SASSTTSSQS GSGTSRKKSI PLSIKNLKRK HKRKKNKITR DFKPGDRVAV 60
EVVTTMTSAD VMWQDGSVEC NIRSNDLFPV HHLDNNEFCP GDFVVDKRVQ SCPDPAVYGV 120
VQSGDHIGRT CMVKWFKLRP SGDDVELIGE EEDVSVYDIA DHPDFRFRTT DIVIRIGNTE 180
DGAPHKEDEQ PSVGQVARVD VSSKVEVVWA DNSKTIILPQ HLYNIESEIE ESDYDSVEGS 240
TSGASSDEWE DDSDSWETDN GLVEDEHPKI EEPPIPPLEQ PVAPEDKGVV ISEEAATAAV 300
QGAVAMAAPM AGLMEKAGKD GPPKSFRELK EAIKILESLK NMTVEQLLTG SPTSPTVEPE 360
KPTREKKFLD DIKKLQENLK KTLDNVAIVE EEKMEAVPDV ERKEDKPEGQ SPVKAEWPSE 420
TPVLCQQCGG KPGVTFTSAK GEVFSVLEFA PSNHSFKKIE FQPPEAKKFF STVRKEMALL 480
ATSLPEGIMV KTFEDRMDLF SALIKGPTRT PYEDGLYLFD IQLPNIYPAV PPHFCYLSQC 540
SGRLNPNLYD NGKVCVSLLG TWIGKGTERW TSKSSLLQVL ISIQGLILVN EPYYNEAGFD 600
SDRGLQEGYE NSRCYNEMAL IRVVQSMTQL VRRPPEVFEQ EIRQHFSTGG WRLVNRIESW 660
LETHALLEKA QALPNGVPKA SSSPEPPAVA ELSDSGQQEP EDGGPAPGEA SQGSDSEGGA 720
QGLASASRDH TDQTSETAPD ASVPPSVKPK KRRKSYRSFL PEKSGYPDIG FPLFPLSKGF 780
IKSIRGVLTQ FRAALLEAGM PECTEDK 807 
Gene Ontology
 GO:0016881; F:acid-amino acid ligase activity; IEA:InterPro. 
Interpro
 IPR000608; UBQ-conjugat_E2.
 IPR016135; UBQ-conjugating_enzyme/RWD. 
Pfam
 PF00179; UQ_con 
SMART
  
PROSITE
 PS50127; UBIQUITIN_CONJUGAT_2 
PRINTS