CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006822
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein BOB1 
Protein Synonyms/Alias
 BEM1-binding protein; Growth inhibitory protein 7 
Gene Name
 BOI1 
Gene Synonyms/Alias
 BOB1; GIN7; YBL085W; YBL0717 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
97ESGNSGVKYGNLNDSubiquitination[1]
110DSASNIGKVSSHQQEubiquitination[1]
171EQTLIQEKTRNEENTubiquitination[1]
346SSESSDRKSSSVTTEubiquitination[1]
521SVVYNNHKRTESGSSubiquitination[1]
552SFDEEETKQPSKASRubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Binds to the BEM1 protein. 
Sequence Annotation
 DOMAIN 13 77 SH3.
 DOMAIN 228 292 SAM.
 DOMAIN 776 895 PH.
 MOD_RES 104 104 Phosphoserine.
 MOD_RES 106 106 Phosphoserine.
 MOD_RES 128 128 Phosphoserine.
 MOD_RES 151 151 Phosphothreonine.
 MOD_RES 158 158 Phosphothreonine.
 MOD_RES 209 209 Phosphoserine.
 MOD_RES 393 393 Phosphoserine.
 MOD_RES 412 412 Phosphoserine.
 MOD_RES 525 525 Phosphoserine.
 MOD_RES 528 528 Phosphoserine.
 MOD_RES 589 589 Phosphoserine.
 MOD_RES 590 590 Phosphoserine.
 MOD_RES 593 593 Phosphoserine.
 MOD_RES 644 644 Phosphoserine.
 MOD_RES 655 655 Phosphoserine.
 MOD_RES 919 919 Phosphothreonine.  
Keyword
 Complete proteome; Phosphoprotein; Reference proteome; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 980 AA 
Protein Sequence
MSLEGNTLGK GAKSFPLYIA VNQYSKRMED ELNMKPGDKI KVITDDGEYN DGWYYGRNLR 60
TKEEGLYPAV FTKRIAIEKP ENLHKSPTQE SGNSGVKYGN LNDSASNIGK VSSHQQENRY 120
TSLKSTMSDI DKALEELRSG SVEQEVSKSP TRVPEVSTPQ LQDEQTLIQE KTRNEENTTH 180
DSLFSSTADL NLSSESLKNI SKSNISTKSL EPSSESVRQL DLKMAKSWSP EEVTDYFSLV 240
GFDQSTCNKF KEHQVSGKIL LELELEHLKE LEINSFGIRF QIFKEIRNIK SAIDSSSNKL 300
DADYSTFAFE NQAAQLMPAA TVNRDEIQQQ ISSKCNKLSS ESSDRKSSSV TTELQRPSSV 360
VVNPNFKLHD PAEQILDMTE VPNLFADKDI FESPGRAPKP PSYPSPVQPP QSPSFNNRYT 420
NNNARFPPQT TYPPKNKNPT VYSNGLIPNS STSSDNSTGK FKFPAMNGHD SNSRKTTLTS 480
ATIPSINTVN TDESLPAISN ISSNATSHHP NRNSVVYNNH KRTESGSSFV DLFNRISMLS 540
PVKSSFDEEE TKQPSKASRA VFDSARRKSS YGHSRDASLS EMKKHRRNSS ILSFFSSKSQ 600
SNPTSPTKQT FTIDPAKMTS HSRSQSNSYS HARSQSYSHS RKHSLVTSPL KTSLSPINSK 660
SNIALAHSET PTSSNNKEAV SQPSEGKHKH KHKHKSKHKH KNSSSKDGSS EEKSKKKLFS 720
STKESFVGSK EFKRSPSELT QKSTKSILPR SNAKKQQTSA FTEGIRSITA KESMQTADCS 780
GWMSKKGTGA MGTWKQRFFT LHGTRLSYFT NTNDEKERGL IDITAHRVLP ASDDDRLISL 840
YAASLGKGKY CFKLVPPQPG SKKGLTFTEP RVHYFAVENK SEMKAWLSAI IKATIDIDTS 900
VPVISSYATP TIPLSKAQTL LEEARLQTQL RDAEEEEGRD QFGWDDTQNK RNSNYPIEQD 960
QFETSDYLES SAFEYPGGRL 980 
Gene Ontology
 GO:0005935; C:cellular bud neck; IDA:SGD.
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0043332; C:mating projection tip; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005543; F:phospholipid binding; IDA:SGD.
 GO:0007118; P:budding cell apical bud growth; IGI:SGD.
 GO:0007109; P:cytokinesis, completion of separation; IGI:SGD. 
Interpro
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR001660; SAM.
 IPR013761; SAM/pointed.
 IPR011510; SAM_2.
 IPR001452; SH3_domain. 
Pfam
 PF00169; PH
 PF07647; SAM_2
 PF00018; SH3_1 
SMART
 SM00233; PH
 SM00454; SAM
 SM00326; SH3 
PROSITE
 PS50003; PH_DOMAIN
 PS50105; SAM_DOMAIN
 PS50002; SH3 
PRINTS