CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030927
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Beta-soluble NSF attachment protein 
Protein Synonyms/Alias
 cDNA FLJ52551, highly similar to Beta-soluble NSF attachment protein 
Gene Name
 NAPB 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
114EQSADYYKGEESNSSubiquitination[1, 2, 3, 4, 5, 6]
124ESNSSANKCLLKVAAubiquitination[6]
164PLLKYSAKDYFFKAAacetylation[7]
164PLLKYSAKDYFFKAAubiquitination[1]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 259 AA 
Protein Sequence
MDNAGKEREA VQLMAEAEKR VKASHSFLRG LFGGNTRIEE ACEMYTRAAN MFKMAKNWSE 60
AINCLNAAID IYTDMGRFTI AAKHHITIAE IYETELVDIE KAIAHYEQSA DYYKGEESNS 120
SANKCLLKVA AYAAQLEQYQ KAIEIYEQVG ANTMDNPLLK YSAKDYFFKA ALCHFIVDEL 180
NAKLALEKYE EMFPAFTDSR ECKLLKKLLE AHEEQNSEAY TEAVKEFDSI SRLDQWLTTM 240
LLRIKKSIQG DGEGDGDLK 259 
Gene Ontology
 GO:0006886; P:intracellular protein transport; IEA:InterPro. 
Interpro
 IPR000744; NSF_attach.
 IPR011990; TPR-like_helical. 
Pfam
  
SMART
  
PROSITE
  
PRINTS
 PR00448; NSFATTACHMNT.