CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044771
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cullin 4B, isoform CRA_e 
Protein Synonyms/Alias
 Cullin-4B 
Gene Name
 CUL4B 
Gene Synonyms/Alias
 hCG_40765 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
45PTSAKKRKLNSSSSSubiquitination[1]
145TSQQQQLKNKSILISubiquitination[1, 2]
500VINPEKDKTMVQELLubiquitination[3]
557IAKYVDSKLRAGNKEubiquitination[4]
587IFRFIYGKDVFEAFYubiquitination[3]
595DVFEAFYKKDLAKRLubiquitination[1, 2, 3, 5]
596VFEAFYKKDLAKRLLubiquitination[4, 5]
606AKRLLVGKSASVDAEubiquitination[2, 4]
621KSMLSKLKHECGAAFubiquitination[2, 5]
631CGAAFTSKLEGMFKDubiquitination[2]
637SKLEGMFKDMELSKDubiquitination[3]
696VKLQEIFKTFYLGKHubiquitination[3]
776LQSLACGKARVLAKNubiquitination[4]
787LAKNPKGKDIEDGDKacetylation[5]
849VRIMKMRKTLSHNLLubiquitination[3]
869NQLKFPVKPADLKKRubiquitination[1]
890RDYMERDKENPNQYNubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 900 AA 
Protein Sequence
MKSVCPVTSG FSSPSPSAAA AAQEVRSATD GNTSTTPPTS AKKRKLNSSS SSSSNSSNER 60
EDFDSTSSSS STPPLQPRDS ASPSTSSFCL GVSVAASSHV PIQKKLRFED TLEFVGFDAK 120
MAEESSSSSS SSSPTAATSQ QQQLKNKSIL ISSVASVHHA NGLAKSSTTV SSFANSKPGS 180
AKKLVIKNFK DKPKLPENYT DETWQKLKEA VEAIQNSTSI KYNLEELYQA VENLCSYKIS 240
ANLYKQLRQI CEDHIKAQIH QFREDSLDSV LFLKKIDRCW QNHCRQMIMI RSIFLFLDRT 300
YVLQNSMLPS IWDMGLELFR AHIISDQKVQ NKTIDGILLL IERERNGEAI DRSLLRSLLS 360
MLSDLQIYQD SFEQRFLEET NRLYAAEGQK LMQEREVPEY LHHVNKRLEE EADRLITYLD 420
QTTQKSLIAT VEKQLLGEHL TAILQKGLNN LLDENRIQDL SLLYQLFSRV RGGVQVLLQQ 480
WIEYIKAFGS TIVINPEKDK TMVQELLDFK DKVDHIIDIC FLKNEKFINA MKEAFETFIN 540
KRPNKPAELI AKYVDSKLRA GNKEATDEEL EKMLDKIMII FRFIYGKDVF EAFYKKDLAK 600
RLLVGKSASV DAEKSMLSKL KHECGAAFTS KLEGMFKDME LSKDIMIQFK QYMQNQNVPG 660
NIELTVNILT MGYWPTYVPM EVHLPPEMVK LQEIFKTFYL GKHSGRKLQW QSTLGHCVLK 720
AEFKEGKKEL QVSLFQTLVL LMFNEGEEFS LEEIKQATGI EDGELRRTLQ SLACGKARVL 780
AKNPKGKDIE DGDKFICNDD FKHKLFRIKI NQIQMKETVE EQASTTERVF QDRQYQIDAA 840
IVRIMKMRKT LSHNLLVSEV YNQLKFPVKP ADLKKRIESL IDRDYMERDK ENPNQYNYIA 900 
Gene Ontology
 GO:0031461; C:cullin-RING ubiquitin ligase complex; IEA:InterPro.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR016157; Cullin_CS.
 IPR016158; Cullin_homology.
 IPR001373; Cullin_N.
 IPR019559; Cullin_neddylation_domain.
 IPR016159; Cullin_repeat-like_dom.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00888; Cullin
 PF10557; Cullin_Nedd8 
SMART
 SM00182; CULLIN
 SM00884; Cullin_Nedd8 
PROSITE
 PS01256; CULLIN_1
 PS50069; CULLIN_2 
PRINTS