UniProt Accession

 VPS21_YEAST; P36017; D6W2F0 

Genbank Protein ID

 X76173; Z29338; X94335; Z74997; BK006948 

Genbank Nucleotide ID

 CAA53769.1; CAA82543.1; CAA64010.1; CAA99285.1; DAA10866.1 

Protein Name

 Vacuolar protein sorting-associated protein 21 

Protein Synonyms/Alias

 GTP-binding protein YPT51; Vacuolar protein-targeting protein 12 

Gene Name

 VPS21 

Gene Synonyms/Alias

 VPS12; VPT12; YPT21; YPT51; YOR089C; YOR3154C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
36	SNDFAENKEPTIGAA	ubiquitination	[1]
103	IKARHWVKELHEQAS	acetylation	[2]
140	VAREEGEKLAEEKGL	ubiquitination	[1]
174	IGEKIPLKTAEEQNS	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Required for protein transport to the vacuole. Involved in two vesicle trafficking steps to the prevacuolar compartment (PVC), regulating the docking of endosomes and Golgi vesicles to the PVC by interacting with PEP7/VAC1 on the PVC membrane and promoting SNARE complex formation. 

Sequence Annotation

 NP_BIND 14 21 GTP.
 NP_BIND 62 66 GTP.
 NP_BIND 120 123 GTP.
 MOTIF 36 44 Effector region (Potential).
 MOD_RES 188 188 Phosphoserine.
 MOD_RES 210 210 Cysteine methyl ester (By similarity).
 LIPID 208 208 S-geranylgeranyl cysteine (By
 LIPID 210 210 S-geranylgeranyl cysteine (By  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; Endosome; GTP-binding; Lipoprotein; Membrane; Methylation; Mitochondrion; Nucleotide-binding; Phosphoprotein; Prenylation; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 210 AA 

Protein Sequence

Gene Ontology

 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005770; C:late endosome; IMP:SGD.
 GO:0005741; C:mitochondrial outer membrane; IDA:SGD.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IDA:SGD.
 GO:0006897; P:endocytosis; IMP:SGD.
 GO:0006895; P:Golgi to endosome transport; IMP:SGD.
 GO:0032511; P:late endosome to vacuole transport via multivesicular body sorting pathway; IGI:SGD.
 GO:0036258; P:multivesicular body assembly; IGI:SGD.
 GO:0036010; P:protein localization to endosome; IGI:SGD.
 GO:0006623; P:protein targeting to vacuole; IMP:SGD.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
 GO:0000011; P:vacuole inheritance; IMP:SGD. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR003579; Small_GTPase_Rab_type. 

Pfam

 PF00071; Ras 

SMART

 SM00175; RAB 

PROSITE

 PS51419; RAB 

PRINTS

 PR00449; RASTRNSFRMNG.