| Tag | Content |
|---|
| CPLM ID | CPLM-006339 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | tRNA (uracil(54)-C(5))-methyltransferase |
Protein Synonyms/Alias | Transfer RNA methyltransferase 2; tRNA(m5U54)-methyltransferase |
Gene Name | TRM2 |
Gene Synonyms/Alias | NUC2; NUD1; RNC1; YKR056W |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 531 | SFAEKNAKANGVENC | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNA. May also have a role in tRNA stabilization or maturation. |
Sequence Annotation | DOMAIN 163 228 TRAM.
ACT_SITE 591 591 Nucleophile (By similarity).
BINDING 461 461 S-adenosyl-L-methionine (By similarity).
BINDING 496 496 S-adenosyl-L-methionine; via carbonyl
BINDING 517 517 S-adenosyl-L-methionine (By similarity).
BINDING 564 564 S-adenosyl-L-methionine (By similarity).
MOD_RES 92 92 Phosphoserine.
MOD_RES 93 93 Phosphoserine. |
Keyword | Complete proteome; Methyltransferase; Phosphoprotein; Reference proteome; S-adenosyl-L-methionine; Transferase; tRNA processing. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 639 AA |
Protein Sequence | MYEQFEFSFF FFENSDNKVK YKAHLISSIK RWSIITCMRC FWTVQKSIFK ARFFACRNFV 60
KKHNYKLIST MTGSTEMVPP TMKHTVDNKR LSSPLTDSGN RRTKKPKLRK YKAKKVETTS 120
PMGVLEFEVN DLLKSQNLSR EQVLNDVTSI LNDKSSTDGP IVLQYHREVK NVKVLEITSN 180
GNGLALIDNP VETEKKQVVI IPFGLPGDVV NIKVFKTHPY YVESDLLDVV EKSPMRRDDL 240
IRDKYFGKSS GSQLEFLTYD DQLELKRKTI MNAYKFFAPR LVAEKLLPPF DTTVASPLQF 300
GYRTKITPHF DMPKRKQKEL SVRPPLGFGQ KGRPQWRKDT LDIGGHGSIL DIDECVLATE 360
VLNKGLTNER RKFEQEFKNY KKGATILLRE NTTILDPSKP TLEQLTEEAS RDENGDISYV 420
EVEDKKNNVR LAKTCVTNPR QIVTEYVDGY TFNFSAGEFF QNNNSILPIV TKYVRDNLQA 480
PAKGDDNKTK FLVDAYCGSG LFSICSSKGV DKVIGVEISA DSVSFAEKNA KANGVENCRF 540
IVGKAEKLFE SIDTPSENTS VILDPPRKGC DELFLKQLAA YNPAKIIYIS CNVHSQARDV 600
EYFLKETENG SAHQIESIRG FDFFPQTHHV ESVCIMKRI 639 |
Gene Ontology | GO:0051908; F:double-stranded DNA specific 5'-3' exodeoxyribonuclease activity; IDA:SGD. GO:0030697; F:S-adenosylmethionine-dependent tRNA (m5U54) methyltransferase activity; IEA:EC. GO:0000014; F:single-stranded DNA specific endodeoxyribonuclease activity; IDA:SGD. GO:0030696; F:tRNA (m5U54) methyltransferase activity; IDA:SGD. GO:0006302; P:double-strand break repair; IMP:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |