CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007021
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ADP-ribosylation factor-binding protein GGA2 
Protein Synonyms/Alias
 Golgi-localized, gamma ear-containing, ARF-binding protein 2 
Gene Name
 GGA2 
Gene Synonyms/Alias
 YHR108W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
18LSELPVRKPQALGNPubiquitination[1]
70DAAIALAKLINNRESacetylation[2]
148ICKHSSYKNDMGYIRacetylation[2]
180ESDLAVLKPSNQLKTacetylation[2]
180ESDLAVLKPSNQLKTubiquitination[1, 3]
193KTASEIQKEQEIAQAubiquitination[1]
229MKIMAGFKEDNAVQAubiquitination[1]
237EDNAVQAKQAISSELubiquitination[1]
246AISSELNKLKRKADLubiquitination[1]
287VAQPKFQKIIEEEQEubiquitination[1, 3]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 May play a role in the regulation of membrane traffic through the trans-Golgi network. 
Sequence Annotation
 DOMAIN 33 169 VHS.
 DOMAIN 196 321 GAT.
 DOMAIN 466 581 GAE.  
Keyword
 3D-structure; Coiled coil; Complete proteome; Golgi apparatus; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 585 AA 
Protein Sequence
MSHPHSHSIY LSELPVRKPQ ALGNPLLRKI QRACRMSLAE PDLALNLDIA DYINEKQGAA 60
PRDAAIALAK LINNRESHVA IFALSLLDVL VKNCGYPFHL QISRKEFLNE LVKRFPGHPP 120
LRYSKIQRLI LTAIEEWYQT ICKHSSYKND MGYIRDMHRL LKYKGYAFPK ISESDLAVLK 180
PSNQLKTASE IQKEQEIAQA AKLEELIRRG KPEDLREANK LMKIMAGFKE DNAVQAKQAI 240
SSELNKLKRK ADLLNEMLES PDSQNWDNET TQELHSALKV AQPKFQKIIE EEQEDDALVQ 300
DLLKFNDTVN QLLEKFNLLK NGDSNAASQI HPSHVSAPLQ QSSGALTNEI NLIDFNDLDE 360
APSQGNNNTN GTGTPAAAET SVNDLLGDLT DLSISNPSTA NQASFGLGGD IVLGSSQPAP 420
PVTTTNNSNN TLDLLGLSTP QSPTNSQAVN SSGFDLLMGF NPTTGTTTAP ARTLVNQSPN 480
LKIEFEISRE SNSVIRIKSF FTNLSSSPIS NLVFLLAVPK SMSLKLQPQS SNFMIGNAKD 540
GISQEGTIEN APANPSKALK VKWKVNYSVN STQAEETAVF TLPNV 585 
Gene Ontology
 GO:0030131; C:clathrin adaptor complex; IEA:InterPro.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0005802; C:trans-Golgi network; IDA:SGD.
 GO:0070273; F:phosphatidylinositol-4-phosphate binding; IDA:SGD.
 GO:0043130; F:ubiquitin binding; IDA:SGD.
 GO:0006895; P:Golgi to endosome transport; IMP:SGD.
 GO:0006896; P:Golgi to vacuole transport; IMP:SGD.
 GO:0043328; P:protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IMP:SGD. 
Interpro
 IPR008152; Clathrin_a/b/g-adaptin_app_Ig.
 IPR008153; Clathrin_g-adaptin_app.
 IPR013041; Coatomer/clathrin_app_Ig-like.
 IPR008942; ENTH_VHS.
 IPR004152; GAT.
 IPR002014; VHS.
 IPR018205; VHS_subgr. 
Pfam
 PF02883; Alpha_adaptinC2
 PF03127; GAT
 PF00790; VHS 
SMART
 SM00809; Alpha_adaptinC2
 SM00288; VHS 
PROSITE
 PS50180; GAE
 PS50909; GAT
 PS50179; VHS 
PRINTS