CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012381
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Membrane-bound transcription factor site-1 protease 
Protein Synonyms/Alias
 Endopeptidase S1P; Subtilisin/kexin-isozyme 1; SKI-1 
Gene Name
 MBTPS1 
Gene Synonyms/Alias
 KIAA0091; S1P; SKI1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
78SFISSALKSSEVDNWubiquitination[1]
107DFEVIQIKEKQKAGLubiquitination[2]
109EVIQIKEKQKAGLLTubiquitination[1]
224FDTGLSEKHPHFKNVubiquitination[1]
388PGGYGRMKPDIVTYGubiquitination[1]
443LVNPASMKQALIASAubiquitination[1, 2]
646PRDNLRMKNDPLDWNubiquitination[1]
934CPRLSWAKPQPLNETubiquitination[1]
951SNLWKHQKLLSIDLDubiquitination[1, 3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Catalyzes the first step in the proteolytic activation of the sterol regulatory element-binding proteins (SREBPs). Other known substrates are BDNF and ATF6. Cleaves after hydrophobic or small residues, provided that Arg or Lys is in position P4. Cleaves known substrates after Arg-Ser-Val-Leu (SERBP-2), Arg-His- Leu-Leu (ATF6), Arg-Gly-Leu-Thr (BDNF) and its own propeptide after Arg-Arg-Leu-Leu. 
Sequence Annotation
 REGION 218 414 Serine protease.
 ACT_SITE 218 218 Charge relay system (By similarity).
 ACT_SITE 249 249 Charge relay system (By similarity).
 ACT_SITE 414 414 Charge relay system (By similarity).
 CARBOHYD 236 236 N-linked (GlcNAc...) (Potential).
 CARBOHYD 305 305 N-linked (GlcNAc...) (Potential).
 CARBOHYD 515 515 N-linked (GlcNAc...) (Potential).
 CARBOHYD 728 728 N-linked (GlcNAc...) (Potential).
 CARBOHYD 939 939 N-linked (GlcNAc...) (Potential).  
Keyword
 Autocatalytic cleavage; Calcium; Cholesterol metabolism; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Glycoprotein; Golgi apparatus; Hydrolase; Lipid metabolism; Membrane; Polymorphism; Protease; Reference proteome; Serine protease; Signal; Steroid metabolism; Sterol metabolism; Transmembrane; Transmembrane helix; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1052 AA 
Protein Sequence
MKLVNIWLLL LVVLLCGKKH LGDRLEKKSF EKAPCPGCSH LTLKVEFSST VVEYEYIVAF 60
NGYFTAKARN SFISSALKSS EVDNWRIIPR NNPSSDYPSD FEVIQIKEKQ KAGLLTLEDH 120
PNIKRVTPQR KVFRSLKYAE SDPTVPCNET RWSQKWQSSR PLRRASLSLG SGFWHATGRH 180
SSRRLLRAIP RQVAQTLQAD VLWQMGYTGA NVRVAVFDTG LSEKHPHFKN VKERTNWTNE 240
RTLDDGLGHG TFVAGVIASM RECQGFAPDA ELHIFRVFTN NQVSYTSWFL DAFNYAILKK 300
IDVLNLSIGG PDFMDHPFVD KVWELTANNV IMVSAIGNDG PLYGTLNNPA DQMDVIGVGG 360
IDFEDNIARF SSRGMTTWEL PGGYGRMKPD IVTYGAGVRG SGVKGGCRAL SGTSVASPVV 420
AGAVTLLVST VQKRELVNPA SMKQALIASA RRLPGVNMFE QGHGKLDLLR AYQILNSYKP 480
QASLSPSYID LTECPYMWPY CSQPIYYGGM PTVVNVTILN GMGVTGRIVD KPDWQPYLPQ 540
NGDNIEVAFS YSSVLWPWSG YLAISISVTK KAASWEGIAQ GHVMITVASP AETESKNGAE 600
QTSTVKLPIK VKIIPTPPRS KRVLWDQYHN LRYPPGYFPR DNLRMKNDPL DWNGDHIHTN 660
FRDMYQHLRS MGYFVEVLGA PFTCFDASQY GTLLMVDSEE EYFPEEIAKL RRDVDNGLSL 720
VIFSDWYNTS VMRKVKFYDE NTRQWWMPDT GGANIPALNE LLSVWNMGFS DGLYEGEFTL 780
ANHDMYYASG CSIAKFPEDG VVITQTFKDQ GLEVLKQETA VVENVPILGL YQIPAEGGGR 840
IVLYGDSNCL DDSHRQKDCF WLLDALLQYT SYGVTPPSLS HSGNRQRPPS GAGSVTPERM 900
EGNHLHRYSK VLEAHLGDPK PRPLPACPRL SWAKPQPLNE TAPSNLWKHQ KLLSIDLDKV 960
VLPNFRSNRP QVRPLSPGES GAWDIPGGIM PGRYNQEVGQ TIPVFAFLGA MVVLAFFVVQ 1020
INKAKSRPKR RKPRVKRPQL MQQVHPPKTP SV 1052 
Gene Ontology
 GO:0005788; C:endoplasmic reticulum lumen; TAS:ProtInc.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0000139; C:Golgi membrane; TAS:Reactome.
 GO:0005795; C:Golgi stack; IEA:Compara.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0004252; F:serine-type endopeptidase activity; IBA:RefGenome.
 GO:0006987; P:activation of signaling protein activity involved in unfolded protein response; TAS:Reactome.
 GO:0044267; P:cellular protein metabolic process; TAS:Reactome.
 GO:0008203; P:cholesterol metabolic process; IEA:UniProtKB-KW.
 GO:0006629; P:lipid metabolic process; IBA:RefGenome.
 GO:0006508; P:proteolysis; IBA:RefGenome.
 GO:0042990; P:regulation of transcription factor import into nucleus; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR000209; Peptidase_S8/S53_dom.
 IPR022398; Peptidase_S8_His-AS.
 IPR023828; Peptidase_S8_Ser-AS.
 IPR015500; Peptidase_S8_subtilisin-rel. 
Pfam
 PF00082; Peptidase_S8 
SMART
  
PROSITE
 PS00136; SUBTILASE_ASP
 PS00137; SUBTILASE_HIS
 PS00138; SUBTILASE_SER 
PRINTS
 PR00723; SUBTILISIN.