UniProt Accession

 MBD2_HUMAN; Q9UBB5; O95242; Q9UIS8 

Genbank Protein ID

 AF072242; AF072246; AF120989; AF120988; AF120993; AF120988; AF120989; AF120990; AF120991; AF120992; BC032638 

Genbank Nucleotide ID

 AAC68871.1; AAC68875.1; AAD56596.1; AAD56596.1; AAD56597.1; AAD56597.1; AAD56597.1; AAD56597.1; AAD56597.1; AAD56597.1; AAH32638.1 

Protein Name

 Methyl-CpG-binding domain protein 2 

Protein Synonyms/Alias

 Demethylase; DMTase; Methyl-CpG-binding protein MBD2 

Gene Name

 MBD2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
186	YFSPSGKKFRSKPQL	ubiquitination	[1]
381	ERVQQVRKKLEEALM	ubiquitination	[1]
382	RVQQVRKKLEEALMA	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters. 

Sequence Annotation

 DOMAIN 145 213 MBD.
 REGION 1 149 Necessary for interaction with DHX9.
 MOD_RES 181 181 Phosphoserine.
 MOD_RES 407 407 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 411 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0000792; C:heterochromatin; IEA:Compara.
 GO:0000118; C:histone deacetylase complex; IEA:Compara.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0008327; F:methyl-CpG binding; IDA:UniProtKB.
 GO:0003729; F:mRNA binding; IEA:Compara.
 GO:0003696; F:satellite DNA binding; TAS:ProtInc.
 GO:0035197; F:siRNA binding; IEA:Compara.
 GO:0043623; P:cellular protein complex assembly; IEA:Compara.
 GO:0042711; P:maternal behavior; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; NAS:UniProtKB.
 GO:0030177; P:positive regulation of Wnt receptor signaling pathway; IEA:Compara.
 GO:0042127; P:regulation of cell proliferation; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR016177; DNA-bd_integrase-typ.
 IPR025884; MeCpG-bd_2/3_C_dom.
 IPR001739; Methyl_CpG_DNA-bd. 

Pfam

 PF01429; MBD
 PF14048; MBD_C 

SMART

 SM00391; MBD 

PROSITE

 PS50982; MBD 

PRINTS