UniProt Accession

 ALKB5_MOUSE; Q3TSG4; Q80US0; Q8BKB9; Q8BKC1 

Genbank Protein ID

 AK053695; AK053700; AK162072; AK163294; AL596386; BC052076 

Genbank Nucleotide ID

 BAC35478.1; BAC35481.1; BAE36711.1; BAE37281.1; CAI35332.1; AAH52076.1 

Protein Name

 RNA demethylase ALKBH5 

Protein Synonyms/Alias

 Alkylated DNA repair protein alkB homolog 5; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5 

Gene Name

 Alkbh5 

Gene Synonyms/Alias

 Abh5; Ofoxd 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
133	DRAPLRNKYFFGEGY	acetylation	[1]
148	TYGAQLQKRGPGQER	ubiquitination	[2]
236	FGCKFQFKPIRVSEP	acetylation	[3, 4]

Reference

 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m(6)A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Requires molecular oxygen, alpha- ketoglutarate and iron (By similarity). Demethylation of m(6)A mRNA affects mRNA processing and export and is required for spermatogenesis. 

Sequence Annotation

 METAL 205 205 Iron; catalytic (By similarity).
 METAL 207 207 Iron; catalytic (By similarity).
 METAL 267 267 Iron; catalytic (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 65 65 Phosphoserine.
 MOD_RES 70 70 Phosphoserine.
 MOD_RES 72 72 Phosphotyrosine (By similarity).
 MOD_RES 133 133 N6-acetyllysine (By similarity).
 MOD_RES 362 362 Phosphoserine (By similarity).
 MOD_RES 372 372 Phosphoserine.
 MOD_RES 375 375 Phosphoserine (By similarity).
 MOD_RES 385 385 Phosphoserine.
 MOD_RES 387 387 Phosphothreonine.  

Keyword

 Acetylation; Coiled coil; Complete proteome; Differentiation; Dioxygenase; Iron; Metal-binding; Nucleus; Oxidoreductase; Phosphoprotein; Reference proteome; Spermatogenesis. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 395 AA 

Protein Sequence

Gene Ontology

 GO:0016607; C:nuclear speck; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0035515; F:oxidative RNA demethylase activity; ISS:UniProtKB.
 GO:0016706; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; ISS:UniProtKB.
 GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:UniProtKB-KW.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0006406; P:mRNA export from nucleus; ISS:UniProtKB.
 GO:0006397; P:mRNA processing; ISS:UniProtKB.
 GO:0035553; P:oxidative single-stranded RNA demethylation; ISS:UniProtKB.
 GO:0001666; P:response to hypoxia; ISS:UniProtKB.
 GO:0007283; P:spermatogenesis; IMP:UniProtKB. 

Interpro

 IPR027450; AlkB-like. 

Pfam

 PF13532; 2OG-FeII_Oxy_2 

SMART

  

PROSITE

  

PRINTS