CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015010
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Large proline-rich protein BAG6 
Protein Synonyms/Alias
 BCL2-associated athanogene 6; HLA-B-associated transcript 3 
Gene Name
 Bag6 
Gene Synonyms/Alias
 Bat3 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
56SVSIPSEKQRLIYQGubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane (By similarity). 
Sequence Annotation
 DOMAIN 17 92 Ubiquitin-like.
 REPEAT 236 265 1.
 REPEAT 410 438 2.
 REPEAT 589 616 3.
 REPEAT 622 650 4.
 REGION 237 650 4 X 29 AA approximate repeats.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 978 978 Phosphoserine (By similarity).
 MOD_RES 987 987 Phosphoserine (By similarity).
 MOD_RES 1095 1095 Phosphoserine (By similarity).
 MOD_RES 1131 1131 Phosphoserine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Apoptosis; Chaperone; Chromatin regulator; Complete proteome; Cytoplasm; Differentiation; Immunity; Nucleus; Phosphoprotein; Reference proteome; Repeat; Spermatogenesis; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1146 AA 
Protein Sequence
MEPSDSTSTA MEEPDSLEVL VKTLDSQTRT FIVGAQMNVK EFKEHIAASV SIPSEKQRLI 60
YQGRVLQDDK KLQDYNVGGK VIHLVERAPP QTQLPSGASS GTGSASATHG GGPLPGTRGP 120
GASGHDRNAN SYVMVGTFNL PSDGSAVDVH INMEQAPIQS EPRVRLVMAQ HMIRDIQTLL 180
SRMECRGGTQ AQASQPPPQT PTVASETVAL NSQTSEPVES EAPPREPMES EEMEERPPTQ 240
TPELPPSGPA PAGPAPAPET NAPNHPSPAE HVEVLQELQR LQRRLQPFLQ RYCEVLGAAA 300
TTDYNNNHEG REEDQRLINL VGESLRLLGN TFVALSDLRC NLACAPPRHL HVVRPMSHYT 360
TPMVLQQAAI PIQINVGTTV TMTGNGARPP PAPGAEAASP GSGQASSLPP SSATVDSSTE 420
GAPPPGPAPP PATSHPRVIR ISHQSVEPVV MMHMNIQDSG AQPGGVPSAP TGPLGPPGHG 480
QSLGQQVPGF PTAPTRVVIA RPTPPQARPS HPGGPPVSGA LQGAGLGTNT SLAQMVSGLV 540
GQLLMQPVLV AQGTPGMAPA SASAPATAQA QAPAPAPAPA PAPATASASA GTTNTATTAG 600
PAPGGPAQPP PPQPSAADLQ FSQLLGNLLG PAGPGAGGPS LASPTITVAV PGVPAFLQGM 660
TEFLQASQAA PPPPPPPPPP PPAPEQQTTP PPGSPSGGTA SPGGLGPESL PPEFFTSVVQ 720
GVLSSLLGSL GARAGSSESI AAFIQRLSGS SNIFEPGADG ALGFFGALLS LLCQNFSMVD 780
VVMLLHGHFQ PLQRLQPQLR SFFHQHYLGG QEPTSSNIRM ATHTLITGLE EYVRESFSLV 840
QVQPGVDIIR TNLEFLQEQF NSIAAHVLHC TDSGFGARLL ELCNQGLFEC LALNLHCLGG 900
QQMELAAVIN GRIRRMSRGV NPSLVSWLTT MMGLRLQVVL EHMPVGPDAI LRYVRRIGDP 960
PQALPEEPME VQGAERTSPE PQREDASPAP GTTAEEAMSR GPPPAPEGGS RDEQDGASAD 1020
AEPWAAAVPP EWVPIIQQDI QSQRKVKPQP PLSDAYLSGM PAKRRKTMQG EGPQLLLSEA 1080
VSRAAKAAGA RPLTSPESLS RDLEAPEVQE SYRQQLRSDI QKRLQEDPNY SPQRFPNAHR 1140
AFADDP 1146 
Gene Ontology
 GO:0071818; C:BAT3 complex; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0031593; F:polyubiquitin binding; ISS:UniProtKB.
 GO:0070628; F:proteasome binding; ISS:UniProtKB.
 GO:0043022; F:ribosome binding; ISS:UniProtKB.
 GO:0007420; P:brain development; ISS:UniProtKB.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0009790; P:embryo development; ISS:UniProtKB.
 GO:0018393; P:internal peptidyl-lysine acetylation; ISS:UniProtKB.
 GO:0042771; P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; ISS:UniProtKB.
 GO:0070059; P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; ISS:UniProtKB.
 GO:0001822; P:kidney development; ISS:UniProtKB.
 GO:0030324; P:lung development; ISS:UniProtKB.
 GO:0032435; P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
 GO:0050821; P:protein stabilization; ISS:UniProtKB.
 GO:0042127; P:regulation of cell proliferation; IEP:RGD.
 GO:0007283; P:spermatogenesis; ISS:UniProtKB.
 GO:0007130; P:synaptonemal complex assembly; ISS:UniProtKB.
 GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; ISS:UniProtKB.
 GO:0006810; P:transport; IEA:UniProtKB-KW.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; ISS:UniProtKB. 
Interpro
 IPR021925; DUF3538.
 IPR000626; Ubiquitin.
 IPR019954; Ubiquitin_CS.
 IPR019955; Ubiquitin_supergroup. 
Pfam
 PF12057; DUF3538
 PF00240; ubiquitin 
SMART
 SM00213; UBQ 
PROSITE
 PS00299; UBIQUITIN_1
 PS50053; UBIQUITIN_2 
PRINTS