CPLM-020858

Genbank Nucleotide ID

Inorganic pyrophosphatase

Protein Synonyms/Alias

Pyrophosphate phospho-hydrolase; PPase

Mus musculus (Mouse)

Position	Peptide	Type	References
25	RVFLKNEKGQYISPF	ubiquitination	[1]
57	VPRWSNAKMEIATKD	acetylation	[2]
57	VPRWSNAKMEIATKD	ubiquitination	[1]
70	KDPLNPIKQDVKKGK	ubiquitination	[1]
170	DPDAANYKDISDVER	acetylation	[3]
170	DPDAANYKDISDVER	ubiquitination	[1]
194	VDWFRRYKVPDGKPE	acetylation	[3, 4, 5, 6]
194	VDWFRRYKVPDGKPE	ubiquitination	[1]
213	FNAEFKNKDFAVDII	acetylation	[2]
213	FNAEFKNKDFAVDII	ubiquitination	[1]
221	DFAVDIIKSTHDYWK	acetylation	[2]
221	DFAVDIIKSTHDYWK	ubiquitination	[1]
228	KSTHDYWKALVTKKT	acetylation	[2, 3, 4, 7, 8]
228	KSTHDYWKALVTKKT	ubiquitination	[1]

[1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
[3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[4] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[5] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[6] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[7] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
[8] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]

Functional Description

METAL 116 116 Magnesium 1 (By similarity).
METAL 121 121 Magnesium 1 (By similarity).
METAL 121 121 Magnesium 2 (By similarity).
METAL 153 153 Magnesium 1 (By similarity).
MOD_RES 2 2 N-acetylserine (By similarity).
MOD_RES 57 57 N6-acetyllysine (By similarity).
MOD_RES 228 228 N6-acetyllysine (By similarity).
MOD_RES 250 250 Phosphoserine (By similarity).

Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Magnesium; Metal-binding; Phosphoprotein; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0004427; F:inorganic diphosphatase activity; IEA:EC.
GO:0000287; F:magnesium ion binding; IEA:InterPro.
GO:0016462; F:pyrophosphatase activity; IDA:MGI.
GO:0006796; P:phosphate-containing compound metabolic process; IC:MGI.

IPR008162; Pyrophosphatase.

PF00719; Pyrophosphatase