CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-036851
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Mitogen-activated protein kinase kinase kinase kinase 4 
Protein Synonyms/Alias
  
Gene Name
 MAP4K4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
261KSKKWSKKFFSFIEGubiquitination[1, 2]
285PSTEQLLKHPFIRDQubiquitination[1, 3, 4]
707KEVFRPLKPADLTALubiquitination[2]
882SASSTLQKHKSSSSFubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1272 AA 
Protein Sequence
MANDSPAKSL VDIDLSSLRD PAGIFELVEV VGNGTYGQVY KGRHVKTGQL AAIKVMDVTE 60
DEEEEIKLEI NMLKKYSHHR NIATYYGAFI KKSPPGHDDQ LWLVMEFCGA GSITDLVKNT 120
KGNTLKEDWI AYISREILRG LAHLHIHHVI HRDIKGQNVL LTENAEVKLV DFGVSAQLDR 180
TVGRRNTFIG TPYWMAPEVI ACDENPDATY DYRSDLWSCG ITAIEMAEGA PPLCDMHPMR 240
ALFLIPRNPP PRLKSKKWSK KFFSFIEGCL VKNYMQRPST EQLLKHPFIR DQPNERQVRI 300
QLKDHIDRTR KKRGEKDETE YEYSGSEEEE EEVPEQEGEP SSIVNVPGES TLRRDFLRLQ 360
QENKERSEAL RRQQLLQEQQ LREQEEYKRQ LLAERQKRIE QQKEQRRRLE EQQRREREAR 420
RQQEREQRRR EQEEKRRLEE LERRRKEEEE RRRAEEEKRR VEREQEYIRR QLEEEQRHLE 480
VLQQQLLQEQ AMLLHDHRRP HPQHSQQPPP PQQERSKPSF HAPEPKAHYE PADRAREVED 540
RFRKTNHSSP EAQSKQTGRV LEPPVPSRSE SFSNGNSESV HPALQRPAEP QVPVRTTSRS 600
PVLSRRDSPL QGSGQQNSQA GQRNSTSIEP RLLWERVEKL VPRPGSGSSS GSSNSGSQPG 660
SHPGSQSGSG ERFRVRSSSK SEGSPSQRLE NAVKKPEDKK EVFRPLKPAD LTALAKELRA 720
VEDVRPPHKV TDYSSSSEES GTTDEEDDDV EQEGADESTS GPEDTRAASS LNLSNGETES 780
VKTMIVHDDV ESEPAMTPSK EGTLIVRQST VDQKRASHHE SNGFAGRIHL LPDLLQQSHS 840
SSTSSTSSSP SSSQPTPTMS PQTPQDKLTA NETQSASSTL QKHKSSSSFT PFIDPRLLQI 900
SPSSGTTVTS VVGFSCDGMR PEAIRQDPTR KGSVVNVNPT NTRPQSDTPE IRKYKKRFNS 960
EILCAALWGV NLLVGTESGL MLLDRSGQGK VYPLINRRRF QQMDVLEGLN VLVTISGKKD 1020
KLRVYYLSWL RNKILHNDPE VEKKQGWTTV GDLEGCVHYK VVKYERIKFL VIALKSSVEV 1080
YAWAPKPYHK FMAFKSFGEL VHKPLLVDLT VEEGQRLKVI YGSCAGFHAV DVDSGSVYDI 1140
YLPTHIQCSI KPHAIIILPN TDGMELLVCY EDEGVYVNTY GRITKDVVLQ WGEMPTSVAY 1200
IRSNQTMGWG EKAIEIRSVE TGHLDGVFMH KRAQRLKFLC ERNDKVFFAS VRSGGSSQVY 1260
FMTLGRTSLL SW 1272 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:InterPro.
 GO:0005083; F:small GTPase regulator activity; IEA:InterPro. 
Interpro
 IPR001180; Citron.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00780; CNH
 PF00069; Pkinase 
SMART
 SM00036; CNH
 SM00220; S_TKc 
PROSITE
 PS50219; CNH
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS